PMID- 7575575
OWN - NLM
STAT- MEDLINE
DCOM- 19951109
LR  - 20081121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 215
IP  - 1
DP  - 1995 Oct 4
TI  - The T-box near the zinc fingers of the human vitamin D receptor is required for 
      heterodimeric DNA binding and transactivation.
PG  - 1-7
AB  - The T-box mediates binding of retinoid X receptor (RXR) homodimers to DNA while 
      the P- and D-box in the zinc fingers of steroid hormone receptors play roles in 
      DNA-binding specificity and homodimerization, respectively. We investigated the 
      function of these elements in the human vitamin D receptor (hVDR) by mutating a 
      Lys-Glu pair of amino acids in the T-box, and by altering the P- and D-boxes to 
      the corresponding residues of the glucocorticoid receptor (GR). The T-box mutant 
      hVDR displayed attenuated vitamin D responsive element (VDRE) binding in the 
      presence of RXR and was severely compromised in transcriptional activation. In 
      contrast, GR P/D-box mutant hVDRs bound to the rat osteocalcin VDRE and elicited 
      near normal transcriptional activation. The T-box mutant uniquely exhibited 
      dominant negative properties, highlighting the significance of this region of 
      hVDR for heterodimeric transcriptional activation.
FAU - Hsieh, J C
AU  - Hsieh JC
AD  - Department of Biochemistry, College of Medicine, University of Arizona, Tucson 
      85724, USA.
FAU - Jurutka, P W
AU  - Jurutka PW
FAU - Selznick, S H
AU  - Selznick SH
FAU - Reeder, M C
AU  - Reeder MC
FAU - Haussler, C A
AU  - Haussler CA
FAU - Whitfield, G K
AU  - Whitfield GK
FAU - Haussler, M R
AU  - Haussler MR
LA  - eng
GR  - AR-15781/AR/NIAMS NIH HHS/United States
GR  - DK-33351/DK/NIDDK NIH HHS/United States
GR  - DK49604-01/DK/NIDDK NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Macromolecular Substances)
RN  - 0 (Receptors, Calcitriol)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - DNA/*metabolism
MH  - Humans
MH  - Macromolecular Substances
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Rats
MH  - Receptors, Calcitriol/*chemistry/genetics/*metabolism
MH  - Structure-Activity Relationship
MH  - *Transcriptional Activation
MH  - Transfection
MH  - *Zinc Fingers
EDAT- 1995/10/04 00:00
MHDA- 1995/10/04 00:01
CRDT- 1995/10/04 00:00
PHST- 1995/10/04 00:00 [pubmed]
PHST- 1995/10/04 00:01 [medline]
PHST- 1995/10/04 00:00 [entrez]
AID - S0006-291X(85)72426-6 [pii]
AID - 10.1006/bbrc.1995.2426 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1995 Oct 4;215(1):1-7. doi: 10.1006/bbrc.1995.2426.