PMID- 7575682
OWN - NLM
STAT- MEDLINE
DCOM- 19951121
LR  - 20190623
IS  - 0006-2952 (Print)
IS  - 0006-2952 (Linking)
VI  - 50
IP  - 7
DP  - 1995 Sep 28
TI  - Ryanodine as inhibitor of chemotactic peptide-induced chemotaxis in human 
      neutrophils.
PG  - 975-9
AB  - Ryanodine gave a moderate inhibition of chemotactic peptide-activated chemotaxis 
      by intact human neutrophils. Chemotaxis by electroporated neutrophils was 
      strongly inhibited in the nanomolar concentration range. Inhibition of chemotaxis 
      by electroporated neutrophils occurs at concentrations known to open calcium 
      channels in ryanodine-sensitive Ca2+ stores. Whereas migration by 
      formyl-methionyl-leucyl-phenylalanine (fMLP)- or interleukin-8-activated 
      electroporated neutrophils was strongly inhibited by ryanodine, chemotaxis 
      induced by protein kinase C activators was not affected. This suggests that the 
      importance of ryanodine-sensitive Ca2+ stores for migration depends on the type 
      of activator used. Ryanodine gave an increase of cytoplasmic free calcium due to 
      the liberation of calcium from internal stores and to the influx of extracellular 
      calcium. The results show that the neutrophil contains ryanodine-sensitive 
      calcium stores that might be involved in receptor-mediated chemotaxis.
FAU - Elferink, J G
AU  - Elferink JG
AD  - Department of Medical Biochemistry, University of Leiden, The Netherlands.
FAU - De Koster, B M
AU  - De Koster BM
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biochem Pharmacol
JT  - Biochemical pharmacology
JID - 0101032
RN  - 0 (Calcium Channels)
RN  - 15662-33-6 (Ryanodine)
RN  - 3G6A5W338E (Caffeine)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Caffeine/pharmacology
MH  - Calcium/metabolism
MH  - Calcium Channels/drug effects
MH  - Cell Movement
MH  - Chemotaxis/*drug effects
MH  - Dose-Response Relationship, Drug
MH  - Electroporation
MH  - Humans
MH  - N-Formylmethionine Leucyl-Phenylalanine/*antagonists & inhibitors/pharmacology
MH  - Neutrophils/*drug effects
MH  - Ryanodine/*pharmacology
EDAT- 1995/09/28 00:00
MHDA- 1995/09/28 00:01
CRDT- 1995/09/28 00:00
PHST- 1995/09/28 00:00 [pubmed]
PHST- 1995/09/28 00:01 [medline]
PHST- 1995/09/28 00:00 [entrez]
AID - 0006-2952(95)00222-L [pii]
AID - 10.1016/0006-2952(95)00222-l [doi]
PST - ppublish
SO  - Biochem Pharmacol. 1995 Sep 28;50(7):975-9. doi: 10.1016/0006-2952(95)00222-l.