PMID- 7577158
OWN - NLM
STAT- MEDLINE
DCOM- 19951212
LR  - 20190904
IS  - 8756-3282 (Print)
IS  - 1873-2763 (Linking)
VI  - 17
IP  - 1
DP  - 1995 Jul
TI  - Cytokine production by calvariae of osteopetrotic mice.
PG  - 5-9
AB  - In the osteopetrotic op/op mouse, the absence of macrophage colony-stimulating 
      factor (M-CSF) prevents the growth of macrophages and osteoclasts and, 
      consequently, bone resorption. In the present study, we investigated whether this 
      deficiency in M-CSF production alters the production of cytokines in op/op bones. 
      Calvariae of phenotypically normal (+/?) and op/op mice were stimulated in vitro 
      with lipopolysaccharide or Pasteurella multocida toxin to produce cytokines. 
      Interleukin-3 (IL-3) and granulocyte-macrophage colony-stimulating factor 
      (GM-CSF) synthesis was the same both in calvaria from osteopetrotic and 
      phenotypically normal animals. However, the production of granulocyte 
      colony-stimulating factor (G-CSF), interleukin-6 (IL-6), and tumor necrosis 
      factor-alpha (TNF alpha) was lower in calvaria from op/op animals than was the 
      case in +/? calvaria. Thus, the lack of biologically active M-CSF causes defects 
      which are not compensated by cells independent of M-CSF.
FAU - Felix, R
AU  - Felix R
AD  - Department of Pathophysiology, University of Berne, Switzerland.
FAU - Genolet, C L
AU  - Genolet CL
FAU - Lowik, C
AU  - Lowik C
FAU - Cecchini, M G
AU  - Cecchini MG
FAU - Hofstetter, W
AU  - Hofstetter W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Bone
JT  - Bone
JID - 8504048
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-3)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Bacterial Toxins/toxicity
MH  - Cell Division/drug effects/physiology
MH  - Cytokines/*biosynthesis
MH  - Disease Models, Animal
MH  - Female
MH  - Granulocyte Colony-Stimulating Factor/biosynthesis
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/biosynthesis
MH  - Interleukin-3/biosynthesis
MH  - Interleukin-6/biosynthesis
MH  - Lipopolysaccharides/toxicity
MH  - Macrophage Colony-Stimulating Factor/*deficiency/genetics/metabolism
MH  - Macrophages/cytology/drug effects
MH  - Male
MH  - Mice
MH  - Osteoclasts/cytology/drug effects
MH  - Osteopetrosis/genetics/*metabolism/physiopathology
MH  - Skull/drug effects/*metabolism
MH  - Tumor Necrosis Factor-alpha/biosynthesis/immunology
EDAT- 1995/07/01 00:00
MHDA- 1995/07/01 00:01
CRDT- 1995/07/01 00:00
PHST- 1995/07/01 00:00 [pubmed]
PHST- 1995/07/01 00:01 [medline]
PHST- 1995/07/01 00:00 [entrez]
AID - 8756328295001306 [pii]
AID - 10.1016/8756-3282(95)00130-6 [doi]
PST - ppublish
SO  - Bone. 1995 Jul;17(1):5-9. doi: 10.1016/8756-3282(95)00130-6.