PMID- 7577649
OWN - NLM
STAT- MEDLINE
DCOM- 19951204
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 91
IP  - 1
DP  - 1995 Sep
TI  - Quantitation of soluble and membrane-bound Fc gamma RIIA (CD32A) mRNA in 
      platelets and megakaryoblastic cell line (Meg-01).
PG  - 37-42
AB  - Fc gamma receptors (Fc gamma Rs) are glycoproteins on platelet surface that bind 
      IgG-containing immune complexes. However, excessive binding of immune complexes 
      leads to platelet activation and thrombosis or increased platelet clearance and 
      thrombocytopenia. In this study, Fc gamma R transcripts in platelets and 
      megakaryoblastic cell line (Meg-01) were investigated using specifically designed 
      oligonucleotides and a new quantitative in situ hybridization assay. Platelets 
      and Meg-01 cells were found to express only Fc gamma RII transcripts. Of Fc gamma 
      RIIA mRNA isoforms (Fc gamma RIIA, B and C), Fc gamma RIIA mRNA predominates in 
      these cells. Platelets and Meg-01 cells contain both alternative spliced forms of 
      Fc gamma RIIA mRNA, those with and without the transmembrane (TM) exon and both 
      forms were present in near equal amounts. In contrast, Fc gamma RIIA transcript 
      with the TM exon predominates in neutrophils and monocytes, suggesting that the 
      splicing of the TM exon is under lineage-specific control.
FAU - Markovic, B
AU  - Markovic B
AD  - Department of Haematology, School of Pathology, University of New South Wales, 
      Sydney, Australia.
FAU - Wu, Z
AU  - Wu Z
FAU - Chesterman, C N
AU  - Chesterman CN
FAU - Chong, B H
AU  - Chong BH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Antigens, CD)
RN  - 0 (Fc gamma receptor IIA)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, IgG)
SB  - IM
MH  - Antigens, CD/*analysis/genetics
MH  - Base Sequence
MH  - Blood Platelets/*chemistry
MH  - Cell Membrane/metabolism
MH  - Gene Expression
MH  - Humans
MH  - In Situ Hybridization/methods
MH  - Macrophages/metabolism
MH  - Megakaryocytes/*chemistry
MH  - Molecular Sequence Data
MH  - Neutrophils/metabolism
MH  - RNA, Messenger/genetics
MH  - Receptors, IgG/*analysis/genetics
MH  - Solubility
MH  - Tumor Cells, Cultured
EDAT- 1995/09/01 00:00
MHDA- 1995/09/01 00:01
CRDT- 1995/09/01 00:00
PHST- 1995/09/01 00:00 [pubmed]
PHST- 1995/09/01 00:01 [medline]
PHST- 1995/09/01 00:00 [entrez]
AID - 10.1111/j.1365-2141.1995.tb05241.x [doi]
PST - ppublish
SO  - Br J Haematol. 1995 Sep;91(1):37-42. doi: 10.1111/j.1365-2141.1995.tb05241.x.