PMID- 7579186 OWN - NLM STAT- MEDLINE DCOM- 19951204 LR - 20190821 IS - 0167-4412 (Print) IS - 0167-4412 (Linking) VI - 29 IP - 2 DP - 1995 Oct TI - Abundance and half-life of the distinct oat phytochrome A3 and A4 mRNAs. PG - 367-77 AB - Gene-preferential oligonucleotide probes were used to determined the relative abundance and half-lives of distinct oat phytochrome A (PHYA) mRNAs. Oat PHYA mRNAs are highly conserved in the 5'-untranslated region and the coding region, but the 3'-untranslated region has an overall lower sequence conservation and was the source of gene-preferential probes. PHYA3 mRNA was estimated to be ca. 61% of the oat PHYA mRNA pool present in poly(A)+ RNA from dark-grown seedlings. The half-lives for PHYA3 and PHYA4 mRNAs were both estimated to be ca. 30 min, and a similar short half-life was estimated for the average PHYA mRNA. Sequence comparisons of PHYA mRNAs from four grass species identified conserved sequences within the 5'- and 3'-untranslated regions that might be important for PHYA mRNA degradation. FAU - Higgs, D C AU - Higgs DC AD - Department of Botany, Iowa State University, Ames 50011, USA. FAU - Barnes, L J AU - Barnes LJ FAU - Colbert, J T AU - Colbert JT LA - eng SI - GENBANK/L34844 SI - GENBANK/X66784 PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - Plant Mol Biol JT - Plant molecular biology JID - 9106343 RN - 0 (Oligonucleotide Probes) RN - 0 (Phytochrome A) RN - 0 (RNA, Messenger) RN - 11121-56-5 (Phytochrome) SB - IM MH - Avena/*genetics/radiation effects MH - Base Sequence MH - Conserved Sequence MH - Half-Life MH - Light MH - Molecular Sequence Data MH - Oligonucleotide Probes MH - Phytochrome/*genetics MH - Phytochrome A MH - Poaceae/genetics MH - RNA, Messenger/analysis/genetics/*metabolism MH - Sequence Homology, Nucleic Acid MH - Transcription, Genetic/radiation effects EDAT- 1995/10/01 00:00 MHDA- 1995/10/01 00:01 CRDT- 1995/10/01 00:00 PHST- 1995/10/01 00:00 [pubmed] PHST- 1995/10/01 00:01 [medline] PHST- 1995/10/01 00:00 [entrez] AID - 10.1007/BF00043659 [doi] PST - ppublish SO - Plant Mol Biol. 1995 Oct;29(2):367-77. doi: 10.1007/BF00043659.