PMID- 7579344
OWN - NLM
STAT- MEDLINE
DCOM- 19951219
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 86
IP  - 10
DP  - 1995 Nov 15
TI  - The value of flow cytometric analysis of platelet glycoprotein expression of 
      CD34+ cells measured under conditions that prevent P-selectin-mediated binding of 
      platelets.
PG  - 3771-82
AB  - In the present study, we show by adhesion assays and ultrastructural studies that 
      platelets can bind to CD34+ cells from human blood and bone marrow and that this 
      interaction interferes with the accurate detection of endogenously expressed 
      platelet glycoproteins (GPs). The interaction between these cells was found to be 
      reversible, dependent on divalent cations, and mediated by P-selectin. Enzymatic 
      characterization showed the involvement of sialic acid residues, protein(s). The 
      demonstration of mRNA for the P-selectin glycoprotein ligand 1 (PSGL-1) in the 
      CD34+ cells by polymerase chain reaction (PCR) analysis suggests that this 
      molecule is present in these cells. Under conditions that prevent platelet 
      adhesion, a small but distinct subpopulation of CD34+ cells diffusely expressed 
      the platelet GPIIb/IIIa complex. These cells were visualized by immunochemical 
      studies. Furthermore, synthesis of mRNA for GPIIb and GPIIIa by CD34+ cells was 
      shown using PCR analysis. The semiquantitative PCR results show relatively higher 
      amounts of GPIIb mRNA than of PF4 mRNA in CD34+CD41+ cells in comparison with 
      this ratio in platelets. This finding is a strong indication that the PCR results 
      are not caused by contaminating adhering platelets. MoAbs against GPIa GPIb 
      alpha, GPV, P-selectin, and the alpha-chain of the vitronectin receptor did not 
      react with CD34+ cells. The number of CD34+ cells expressing GPIIb/IIIa present 
      in peripheral blood stem cell (PBSC) transplants was determined and was 
      correlated with platelet recovery after intensive chemotherapy in 27 patients. 
      The number of CD34+CD41+ cells correlated significantly better with the time of 
      platelet recovery after PBSC transplantation (r = .83, P = .04) than did the 
      total number of CD34+ cells (r = .55). Statistical analysis produced a threshold 
      value for rapid platelet recovery of 0.34 x 10(6) CD34+CD41+ cells/kg. This study 
      suggests that if performed in the presence of EDTA the flow cytometric 
      measurement of GPIIb/IIIa on CD34+ cells provides the most accurate indication of 
      the platelet reconstitutive capacity of the PBSC transplant.
FAU - Dercksen, M W
AU  - Dercksen MW
AD  - European Cancer Centre, Amsterdam, The Netherlands.
FAU - Weimar, I S
AU  - Weimar IS
FAU - Richel, D J
AU  - Richel DJ
FAU - Breton-Gorius, J
AU  - Breton-Gorius J
FAU - Vainchenker, W
AU  - Vainchenker W
FAU - Slaper-Cortenbach, C M
AU  - Slaper-Cortenbach CM
FAU - Pinedo, H M
AU  - Pinedo HM
FAU - von dem Borne, A E
AU  - von dem Borne AE
FAU - Gerritsen, W R
AU  - Gerritsen WR
FAU - van der Schoot, C E
AU  - van der Schoot CE
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD34)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (P-Selectin)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (Recombinant Proteins)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
RN  - 9G34HU7RV0 (Edetic Acid)
RN  - EC 3.2.1.18 (Neuraminidase)
RN  - EC 3.4.21.36 (Pancreatic Elastase)
RN  - EC 3.4.21.4 (Trypsin)
RN  - EC 3.4.21.5 (Thrombin)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.57 (O-sialoglycoprotein endopeptidase)
RN  - PVI5M0M1GW (Filgrastim)
SB  - IM
MH  - Antigens, CD34/*analysis
MH  - Antigens, Differentiation/*analysis
MH  - Antineoplastic Combined Chemotherapy Protocols/pharmacology/therapeutic use
MH  - *Artifacts
MH  - Base Sequence
MH  - Blood Cells
MH  - Bone Marrow/pathology
MH  - Cell Differentiation
MH  - Edetic Acid/pharmacology
MH  - Filgrastim
MH  - *Flow Cytometry
MH  - Granulocyte Colony-Stimulating Factor/pharmacology
MH  - Heart Diseases/pathology/surgery
MH  - Hematopoiesis/drug effects
MH  - Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - Immunophenotyping
MH  - Megakaryocytes/chemistry/*cytology
MH  - Metalloendopeptidases/pharmacology
MH  - Molecular Sequence Data
MH  - Neoplasms/blood/drug therapy/pathology/therapy
MH  - Neuraminidase/pharmacology
MH  - *P-Selectin
MH  - Pancreatic Elastase/pharmacology
MH  - Platelet Activation/drug effects
MH  - Platelet Adhesiveness/*drug effects
MH  - Platelet Membrane Glycoproteins/*biosynthesis/genetics
MH  - Polymerase Chain Reaction
MH  - Recombinant Proteins/pharmacology
MH  - Thrombin/pharmacology
MH  - Trypsin/pharmacology
EDAT- 1995/11/15 00:00
MHDA- 1995/11/15 00:01
CRDT- 1995/11/15 00:00
PHST- 1995/11/15 00:00 [pubmed]
PHST- 1995/11/15 00:01 [medline]
PHST- 1995/11/15 00:00 [entrez]
AID - S0006-4971(20)63228-1 [pii]
PST - ppublish
SO  - Blood. 1995 Nov 15;86(10):3771-82.