PMID- 7581011
OWN - NLM
STAT- MEDLINE
DCOM- 19951222
LR  - 20191210
IS  - 1039-9712 (Print)
IS  - 1039-9712 (Linking)
VI  - 36
IP  - 5
DP  - 1995 Aug
TI  - Intracellular Ca2+ pool depletion is linked to the induction of nitric oxide 
      synthesis in murine peritoneal macrophages.
PG  - 949-55
AB  - The ability of putative Ca(2+)-ATPase inhibitor of endoplasmic reticulum (ER), 
      thapsigargin (TG), to induce nitric oxide (NO) synthesis in murine peritoneal 
      macrophages was examined. TG alone had small effect on NO synthesis, whereas TG 
      in combination with LPS markedly increased NO synthesis in a dose dependent 
      manner. This increase in NO synthesis was reflected as increased amount of 
      inducible NO synthase (iNOS) mRNA by Northern blotting. In addition, the ability 
      of TG on NO synthesis could be mimicked by another chemically unrelated inhibitor 
      of Ca(2+)-ATPase, 2,5-DI-(t-butyl)-1, 4-benzohydroquinone (tBuBHQ). Adding EGTA, 
      a calcium chelator, to the incubation medium significantly reduced the ability of 
      macrophages to induce NO synthesis in response to the optimal stimulation of TG 
      or TG plus LPS. These results therefore demonstrate that intracellular Ca2+ pool 
      depletion is linked to the induction of NO synthesis in murine peritoneal 
      macrophages and further suggest that it is also related with interferon-gamma 
      (IFN-gamma)-induced signaling.
FAU - Park, Y C
AU  - Park YC
AD  - Department of Molecular Biology, College of Natural Sciences, Pusan National 
      University, Korea.
FAU - Jun, C D
AU  - Jun CD
FAU - Kang, H S
AU  - Kang HS
FAU - Kim, H D
AU  - Kim HD
FAU - Kim, H M
AU  - Kim HM
FAU - Chung, H T
AU  - Chung HT
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biochem Mol Biol Int
JT  - Biochemistry and molecular biology international
JID - 9306673
RN  - 0 (Chelating Agents)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Hydroquinones)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Terpenes)
RN  - 26XK13B61B (2,5-di-tert-butylhydroquinone)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 526U7A2651 (Egtazic Acid)
RN  - 67526-95-8 (Thapsigargin)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Calcium-Transporting ATPases/antagonists & inhibitors
MH  - Cells, Cultured
MH  - Chelating Agents/pharmacology
MH  - Egtazic Acid/pharmacology
MH  - Endoplasmic Reticulum/enzymology
MH  - Enzyme Induction
MH  - Enzyme Inhibitors/pharmacology
MH  - Female
MH  - Hydroquinones/pharmacology
MH  - Interferon-gamma/pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophage Activation
MH  - Macrophages, Peritoneal/enzymology/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Nitric Oxide/*biosynthesis
MH  - Nitric Oxide Synthase/*biosynthesis
MH  - RNA, Messenger/biosynthesis
MH  - Terpenes/pharmacology
MH  - Thapsigargin
EDAT- 1995/08/01 00:00
MHDA- 1995/08/01 00:01
CRDT- 1995/08/01 00:00
PHST- 1995/08/01 00:00 [pubmed]
PHST- 1995/08/01 00:01 [medline]
PHST- 1995/08/01 00:00 [entrez]
PST - ppublish
SO  - Biochem Mol Biol Int. 1995 Aug;36(5):949-55.