PMID- 7581376
OWN - NLM
STAT- MEDLINE
DCOM- 19951215
LR  - 20190512
IS  - 0964-6906 (Print)
IS  - 0964-6906 (Linking)
VI  - 4
IP  - 8
DP  - 1995 Aug
TI  - Isolation of chromosome-specific genes by reciprocal probing of arrayed cDNA and 
      cosmid libraries.
PG  - 1373-80
AB  - We have identified and mapped 61 novel and previously described chromosome 17 and 
      X genes, using a human placental cDNA library. These genes were isolated using a 
      gene identification and mapping strategy based on reciprocal probing of arrayed 
      chromosome specific cosmid and cDNA libraries. This strategy scans gridded 
      cosmids for nuclear genes and isolates the expressed sequence by a cosmid to cDNA 
      filter hybridization. Inherent to this approach is the advantage of identifying 
      the corresponding genomic cosmid clone of a particular cDNA. The genomic and cDNA 
      reagents can be used for fluorescence in situ hybridization (FISH) and polymerase 
      chain reaction (PCR) based mapping to resolve map positions of cDNAs belonging to 
      gene families and those associated with multiple chromosomes. The downstream 
      utility of reagents generated by the reciprocal probing methods is demonstrated 
      in our studies.
FAU - Lee, C C
AU  - Lee CC
AD  - Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, 
      TX 77030 USA.
FAU - Yazdani, A
AU  - Yazdani A
FAU - Wehnert, M
AU  - Wehnert M
FAU - Zhao, Z Y
AU  - Zhao ZY
FAU - Lindsay, E A
AU  - Lindsay EA
FAU - Bailey, J
AU  - Bailey J
FAU - Coolbaugh, M I
AU  - Coolbaugh MI
FAU - Couch, L
AU  - Couch L
FAU - Xiong, M
AU  - Xiong M
FAU - Chinault, A C
AU  - Chinault AC
AU  - et al.
LA  - eng
SI  - GENBANK/L31976
SI  - GENBANK/L31991
SI  - GENBANK/L32048
SI  - GENBANK/L32058
SI  - GENBANK/L32065
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Hum Mol Genet
JT  - Human molecular genetics
JID - 9208958
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
SB  - IM
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 17/*genetics
MH  - Cosmids
MH  - DNA Primers/genetics
MH  - DNA, Complementary/*genetics
MH  - Female
MH  - Gene Library
MH  - Humans
MH  - Molecular Sequence Data
MH  - Pregnancy
MH  - X Chromosome/*genetics
EDAT- 1995/08/01 00:00
MHDA- 1995/08/01 00:01
CRDT- 1995/08/01 00:00
PHST- 1995/08/01 00:00 [pubmed]
PHST- 1995/08/01 00:01 [medline]
PHST- 1995/08/01 00:00 [entrez]
AID - 10.1093/hmg/4.8.1373 [doi]
PST - ppublish
SO  - Hum Mol Genet. 1995 Aug;4(8):1373-80. doi: 10.1093/hmg/4.8.1373.