PMID- 7581869 OWN - NLM STAT- MEDLINE DCOM- 19951212 LR - 20190913 IS - 1572-6495 (Print) IS - 1572-6495 (Linking) VI - 668 IP - 2 DP - 1995 Jun 23 TI - Selective removal of immunoglobulin E from rat blood by membrane-immobilized antibody. PG - 327-32 AB - We examined the suitability of an immunoaffinity membrane [rabbit IgG specific for rat immunoglobulin E (IgE) immobilized on a cellulose membrane] for removing IgE from rat blood passed through a simple extracorporeal circulatory system. To determine the concentration of IgE in the blood, we also developed a highly sensitive chemiluminescence enzyme immunoassay for rat IgE. The IgE levels in the outlet blood from the immunoaffinity membrane module decreased to 30% of the initial concentration within 30 min. FAU - Adachi, T AU - Adachi T AD - Hatano Research Institute, Food and Drug Safety Center, Kanagawa, Japan. FAU - Mogi, M AU - Mogi M FAU - Harada, M AU - Harada M FAU - Kojima, K AU - Kojima K LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - J Chromatogr B Biomed Appl JT - Journal of chromatography. B, Biomedical applications JID - 9421796 RN - 37341-29-0 (Immunoglobulin E) SB - IM MH - Animals MH - Enzyme-Linked Immunosorbent Assay/*methods MH - Evaluation Studies as Topic MH - Immunoglobulin E/*blood/immunology MH - Luminescent Measurements MH - Models, Biological MH - Rats EDAT- 1995/06/23 00:00 MHDA- 1995/06/23 00:01 CRDT- 1995/06/23 00:00 PHST- 1995/06/23 00:00 [pubmed] PHST- 1995/06/23 00:01 [medline] PHST- 1995/06/23 00:00 [entrez] AID - 037843479500095Z [pii] AID - 10.1016/0378-4347(95)00095-z [doi] PST - ppublish SO - J Chromatogr B Biomed Appl. 1995 Jun 23;668(2):327-32. doi: 10.1016/0378-4347(95)00095-z.