PMID- 7585610
OWN - NLM
STAT- MEDLINE
DCOM- 19951215
LR  - 20220223
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 55
IP  - 22
DP  - 1995 Nov 15
TI  - Chromosomal anomalies in prostatic intraepithelial neoplasia and carcinoma 
      detected by fluorescence in situ hybridization.
PG  - 5408-14
AB  - The pathogenetic relationship between high-grade prostatic intraepithelial 
      neoplasia (PIN), prostatic carcinoma, and metastases is poorly understood. We 
      used fluorescence in situ hybridization (FISH) with centromere-specific probes 
      for chromosomes 7, 8, 10, 12, and Y to evaluate numeric chromosomal anomalies in 
      PIN (68 foci), localized prostatic carcinoma (78 foci), and lymph node metastases 
      (8 foci) in 40 whole-mount radical prostatectomy and pelvic lymphadenectomy 
      specimens. Chromosomal anomalies were found in 50, 51, and 100% of the foci of 
      PIN, carcinoma, and metastases, respectively. The mean numbers of abnormal 
      chromosomes per focus were 0.66 in PIN, 1.09 in carcinoma, and 3.75 in 
      metastases. The most frequent anomaly in PIN was a gain of chromosome 8 (32% of 
      foci), followed by gains of chromosomes 10 (13%), 7 (10%), 12 (4%), and Y (4%). 
      The most frequent anomalies in foci of carcinoma were gains of chromosomes 7 and 
      8 (28% and 30% of foci, respectively), followed by gains of chromosomes 10 (23%), 
      12 (9%), and Y (9%). There was a positive correlation of the gain of chromosome 8 
      with the pathological stage and Gleason score (both P < 0.05). Usually, carcinoma 
      foci contained more anomalies than paired PIN foci, but five prostates contained 
      one or more foci of PIN with more anomalies than carcinoma. Among the cases with 
      metastases, usually one or more foci of the primary tumor shared chromosomal 
      anomalies with the matched metastases. Our results indicate that PIN and 
      prostatic carcinoma foci have similar proportions of chromosomal anomalies, but 
      foci of carcinoma usually have more alterations. This observation supports the 
      hypothesis that PIN is often a precursor of carcinoma, although there are some 
      carcinoma foci that have few or no apparent chromosomal alterations, whereas 
      concurrent PIN foci have multiple alterations. A gain of chromosome 8 was the 
      most common numerical alteration and was associated with increasing cancer stage 
      and grade, suggesting that it may play a role in the initiation and progression 
      of prostatic carcinoma. Usually, one or more foci of the primary tumor shared 
      chromosomal anomalies with associated lymph node metastases, suggesting that, 
      often, just a single focus of carcinoma gives rise to metastases.
FAU - Qian, J
AU  - Qian J
AD  - Department of Laboratory Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA.
FAU - Bostwick, D G
AU  - Bostwick DG
FAU - Takahashi, S
AU  - Takahashi S
FAU - Borell, T J
AU  - Borell TJ
FAU - Herath, J F
AU  - Herath JF
FAU - Lieber, M M
AU  - Lieber MM
FAU - Jenkins, R B
AU  - Jenkins RB
LA  - eng
GR  - CA 58225/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
SB  - IM
MH  - Aged
MH  - *Chromosome Aberrations
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Lymphatic Metastasis
MH  - Male
MH  - Middle Aged
MH  - Prostatic Intraepithelial Neoplasia/*genetics/pathology
MH  - Prostatic Neoplasms/*genetics/pathology
EDAT- 1995/11/15 00:00
MHDA- 1995/11/15 00:01
CRDT- 1995/11/15 00:00
PHST- 1995/11/15 00:00 [pubmed]
PHST- 1995/11/15 00:01 [medline]
PHST- 1995/11/15 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1995 Nov 15;55(22):5408-14.