PMID- 7586307
OWN - NLM
STAT- MEDLINE
DCOM- 19951228
LR  - 20191210
IS  - 0009-7322 (Print)
IS  - 0009-7322 (Linking)
VI  - 92
IP  - 11
DP  - 1995 Dec 1
TI  - Unchanged protein levels of SERCA II and phospholamban but reduced Ca2+ uptake 
      and Ca(2+)-ATPase activity of cardiac sarcoplasmic reticulum from dilated 
      cardiomyopathy patients compared with patients with nonfailing hearts.
PG  - 3220-8
AB  - BACKGROUND: The aim of the present study was to investigate whether Ca2+ uptake 
      into the sarcoplasmic reticulum (SR) is altered in failing human myocardium 
      resulting from dilated cardiomyopathy. METHODS AND RESULTS: Ca(2+)-ATPase (SERCA 
      II) activity and Ca(2+)-dependent 45Ca2+ uptake (oxalate supported, steady state) 
      in isolated vesicles from the SR (VSR) and in crude membrane preparations (CSR) 
      (free Ca2+, 0.01 to 100 mumol/L) from nonfailing (donor hearts, n = 13) and 
      terminally failing (heart transplants, dilated cardiomyopathy, n = 17) human 
      myocardium were studied. In the same hearts, protein levels (Western blot 
      analysis) and mRNA levels (Northern blot analysis) of SERCA II and phospholamban 
      were measured. Increasing concentrations of Ca2+ were followed by an increased 
      Ca(2+)-ATPase activity and Ca2+ uptake. Ca2+ uptake activity and Ca(2+)-ATPase 
      activity in CSR preparations from failing myocardium were significantly reduced 
      compared with nonfailing hearts (Ca(2+)-ATPase, 163 +/- 8 and 125 +/- 7 nmol 
      ATP/mg protein per minute for nonfailing tissue and failing tissue in New York 
      Heart Association [NYHA] class IV, respectively; Ca2+ uptake, 7.1 +/- 0.8 and 3.5 
      +/- 0.3 nmol/mg protein per minute in CSR from nonfailing and NYHA class IV 
      hearts, respectively P < .05). In contrast, no significant difference was 
      measured in VSR. In the same preparations (CSR and VSR), both SERCA II and 
      phospholamban levels (Western blot technique with monoclonal antibodies) were 
      unchanged in failing compared with nonfailing tissue. mRNA expression relative to 
      GAPDH mRNA for SERCA IIa and for phospholamban was significantly reduced in 
      failing human myocardium (P < .05). CONCLUSIONS: These findings provide evidence 
      that in failing human myocardium caused by dilated cardiomyopathy, protein levels 
      of SERCA II and phospholamban are unchanged even though mRNA levels for SERCA II 
      and phospholamban and the SERCA II function are reduced compared with nonfailing 
      myocardium.
FAU - Schwinger, R H
AU  - Schwinger RH
AD  - Universitat zu Koln, Medizinische Klinik III, Germany.
FAU - Bohm, M
AU  - Bohm M
FAU - Schmidt, U
AU  - Schmidt U
FAU - Karczewski, P
AU  - Karczewski P
FAU - Bavendiek, U
AU  - Bavendiek U
FAU - Flesch, M
AU  - Flesch M
FAU - Krause, E G
AU  - Krause EG
FAU - Erdmann, E
AU  - Erdmann E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Circulation
JT  - Circulation
JID - 0147763
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (phospholamban)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adenosine Triphosphatases/*metabolism
MH  - Adult
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Calcium/*metabolism
MH  - Calcium-Binding Proteins/*metabolism
MH  - Calcium-Transporting ATPases/*metabolism
MH  - Cardiomyopathy, Dilated/*metabolism/physiopathology
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Male
MH  - Myocardial Contraction/physiology
MH  - Myocardium/*metabolism
MH  - RNA, Messenger/analysis
MH  - Sarcoplasmic Reticulum/*metabolism
EDAT- 1995/12/01 00:00
MHDA- 1995/12/01 00:01
CRDT- 1995/12/01 00:00
PHST- 1995/12/01 00:00 [pubmed]
PHST- 1995/12/01 00:01 [medline]
PHST- 1995/12/01 00:00 [entrez]
AID - 10.1161/01.cir.92.11.3220 [doi]
PST - ppublish
SO  - Circulation. 1995 Dec 1;92(11):3220-8. doi: 10.1161/01.cir.92.11.3220.