PMID- 7586737
OWN - NLM
STAT- MEDLINE
DCOM- 19951228
LR  - 20190821
IS  - 0090-1229 (Print)
IS  - 0090-1229 (Linking)
VI  - 77
IP  - 3
DP  - 1995 Dec
TI  - A patient with autoimmune thrombocytopenic purpura demonstrating serum antibodies 
      reactive with mouse cross-reactive idiotypes.
PG  - 271-81
AB  - Autoimmune thrombocytopenic purpura (ATP) is a syndrome of destructive 
      thrombocytopenia due to platelet-binding antibodies. We report the case of a 
      young woman (C.V.) who has a history of chronic ATP with severe but transient 
      bouts of thrombocytopenia. Using the classic monoclonal antibody (MAb) 
      immobilization of platelet antigens (MAIPA) assay to screen serum antibody 
      specificity, results were strongly positive with MAbs to glycoproteins (GPs) 
      Ib-IX, Ia-IIa, IV, and p24, but weakly positive or negative for GP IIb-IIIa. In 
      contrast, a two-step incubation assay (MAIPA II), in which platelets were 
      incubated sequentially with C.V. serum and the murine MAb, gave negative results 
      for all GPs. Affinity chromatography performed using Bx-1, a MAb to GP Ib, showed 
      that the patient's serum contained antibodies to determinants expressed by mouse 
      immunoglobulins. These were present on Fab fragments on Bx-1. A survey of sera 
      from other patients with thrombocytopenia of immune origin revealed that 
      antibodies reactive with selected idiotypes of mouse MAbs were not infrequent and 
      raises the question of their role in the thrombocytopenia.
FAU - Clofent-Sanchez, G
AU  - Clofent-Sanchez G
AD  - URA 1464 CNRS, Pathologie Cellulaire de l'Hemostase, Hopital Cardiologique, 
      Pessac, France.
FAU - Laroche-Traineau, J
AU  - Laroche-Traineau J
FAU - Bermudes, H
AU  - Bermudes H
FAU - Lucas, S
AU  - Lucas S
FAU - Nurden, P
AU  - Nurden P
FAU - Nurden, A
AU  - Nurden A
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Clin Immunol Immunopathol
JT  - Clinical immunology and immunopathology
JID - 0356637
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Autoantibodies)
RN  - 0 (Epitopes)
RN  - 0 (Glycoproteins)
RN  - 0 (Immunoglobulin Isotypes)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Antibodies, Anti-Idiotypic/*blood
MH  - Antibodies, Monoclonal/immunology
MH  - Autoantibodies/*blood
MH  - Blood Platelets/immunology
MH  - Cross Reactions
MH  - Epitopes/immunology
MH  - Female
MH  - Glycoproteins/blood/*immunology
MH  - Humans
MH  - Immunoassay/methods
MH  - Immunoglobulin Isotypes/immunology
MH  - Mice
MH  - Middle Aged
MH  - Purpura, Thrombocytopenic, Idiopathic/*immunology/pathology
EDAT- 1995/12/01 00:00
MHDA- 1995/12/01 00:01
CRDT- 1995/12/01 00:00
PHST- 1995/12/01 00:00 [pubmed]
PHST- 1995/12/01 00:01 [medline]
PHST- 1995/12/01 00:00 [entrez]
AID - S0090122985711531 [pii]
AID - 10.1006/clin.1995.1153 [doi]
PST - ppublish
SO  - Clin Immunol Immunopathol. 1995 Dec;77(3):271-81. doi: 10.1006/clin.1995.1153.