PMID- 7587724
OWN - NLM
STAT- MEDLINE
DCOM- 19951227
LR  - 20151119
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 20
IP  - 4
DP  - 1995 Aug 1
TI  - Autofluorescence correction for fluorescence in situ hybridization.
PG  - 356-61
AB  - Optimal sensitivity of fluorescence in situ hybridization (FISH) requires bright 
      signals and low background fluorescence. Use of locus-specific probes is 
      especially dependent on high sensitivity. Some tissue preparations show high 
      autofluorescence, masking small or dim signals. We have developed a new method 
      for subtracting autofluorescence from digital images on a pixel-by-pixel basis. 
      It is based on the observation that fluorescent labels for FISH have narrower 
      excitation and emission spectra than the chemical components responsible for 
      autofluorescence. Our new approach uses calculation of the ratio of 
      autofluorescence between multiple color images for correction of autofluorescence 
      in each individual image. By subtracting autofluorescence components, we were 
      able to enhance centromeric signals and make previously indistinguishable cosmid 
      signals clearly visible. This image-processing approach to autofluorescence 
      correction may widen the applicability of gene-specific probes in FISH analysis 
      of tumor material.
FAU - Szollosi, J
AU  - Szollosi J
AD  - Department of Laboratory Medicine, University of California, San Francisco, USA.
FAU - Lockett, S J
AU  - Lockett SJ
FAU - Balazs, M
AU  - Balazs M
FAU - Waldman, F M
AU  - Waldman FM
LA  - eng
GR  - CA-44768/CA/NCI NIH HHS/United States
GR  - CA-47537/CA/NCI NIH HHS/United States
GR  - CA-49056/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Deoxyuracil Nucleotides)
RN  - 0 (Fluoresceins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Rhodamines)
RN  - 1173-82-6 (deoxyuridine triphosphate)
RN  - 62669-72-1 (tetramethylrhodamine)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
RN  - NQ1SX9LNAU (Digoxigenin)
RN  - TPY09G7XIR (Fluorescein)
SB  - IM
MH  - *Artifacts
MH  - Centromere/ultrastructure
MH  - Chromosomes, Human, Pair 17
MH  - DNA Probes
MH  - DNA, Neoplasm/analysis
MH  - Deoxyuracil Nucleotides
MH  - Digoxigenin
MH  - Fluorescein
MH  - Fluoresceins
MH  - *Fluorescence
MH  - Fluorescent Dyes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Neoplasm Proteins/genetics
MH  - Receptor, ErbB-2/genetics
MH  - Rhodamines
MH  - *Subtraction Technique
MH  - Urinary Bladder Neoplasms/chemistry/genetics
EDAT- 1995/08/01 00:00
MHDA- 1995/08/01 00:01
CRDT- 1995/08/01 00:00
PHST- 1995/08/01 00:00 [pubmed]
PHST- 1995/08/01 00:01 [medline]
PHST- 1995/08/01 00:00 [entrez]
AID - 10.1002/cyto.990200412 [doi]
PST - ppublish
SO  - Cytometry. 1995 Aug 1;20(4):356-61. doi: 10.1002/cyto.990200412.