PMID- 7588230
OWN - NLM
STAT- MEDLINE
DCOM- 19951127
LR  - 20131121
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 136
IP  - 11
DP  - 1995 Nov
TI  - Prostaglandin F2 alpha mediates ovarian sterol carrier protein-2 expression 
      during luteolysis.
PG  - 4963-72
AB  - In the corpus luteum, prostaglandin F2 alpha (PGF2 alpha) appears to be a 
      physiological agent with both antisteroidogenic and luteolytic actions. It is 
      hypothesized that the antisteroidogenic action of PGF2 alpha acts through altered 
      transport of cholesterol to the mitochondrial cytochrome P450 side-chain cleavage 
      enzyme (P450scc). However, the effect of PGF2 alpha on the expression of the 
      putative cholesterol transport protein, sterol carrier protein-2 (SCP2; 13.2 
      kilodaltons), has not been examined. In this study, the decline in serum 
      progesterone after PGF2 alpha injection was examined in parallel with altered 
      ovarian SCP2, P450scc, and 3 beta-hydroxysteroid dehydrogenase (3 beta HSD) 
      protein and messenger RNA (mRNA) levels. Rats (28 days old) were treated with 8 
      IU PMSG to induce follicular development and ovulation. Ten days after ovulation, 
      animals were treated with PGF2 alpha (single or multiple injections; 100-250 
      micrograms each) or left untreated. Ovarian SCP2, P450scc, and 3 beta HSD protein 
      and mRNA levels were examined 0 (time zero), 4, and 8 h post-PGF2 alpha treatment 
      using Western and Northern blot analysis. SCP2 mRNA levels were also examined 
      using a highly sensitive ribonuclease protection assay that detects a 429-base 
      pair SCP2-mRNA specific sequence. The results indicate that serum progesterone 
      was significantly reduced 4 and 8 h after PGF2 alpha injections (P < 0.001; n = 
      6/time point). The decline in progesterone paralleled a 50-60% reduction in 3 
      beta HSD protein and mRNA levels by 4 h post-PGF2 alpha. Protein and mRNA levels 
      for 3 beta HSD returned to control values by 8 h post-PGF2 alpha treatment. 
      P450scc expression was also reduced at 4 h (44-54%), but by 8 h, both protein and 
      mRNA levels had increased above the normal control levels (P < 0.02). In 
      contrast, the 0.8-kilobase SCP2-specific mRNA transcript was reduced to 50% and 
      80% of the pre-PGF2 alpha treatment level at 4 and 8 h, respectively (P < 0.01). 
      SCP2 ribonuclease protection assay analysis also indicated that SCP2 mRNA levels 
      were reduced 65% (P < 0.03) and 85% (P < 0.01) by 4 and 8 h post-PGF2 alpha 
      treatment compared to those in time zero ovarian tissue. Consistent with the loss 
      of SCP2 mRNA expression, Western blot analysis indicated that a 15-kilodalton 
      SCP2-immunoreactive protein (presumably the pro-SCP2 form) was significantly 
      reduced or absent in the PGF2 alpha treated animals (P < 0.04).(ABSTRACT 
      TRUNCATED AT 400 WORDS)
FAU - McLean, M P
AU  - McLean MP
AD  - Department of Obstetrics and Gynecology, University of South Florida College of 
      Medicine, Tampa 33606, USA.
FAU - Billheimer, J T
AU  - Billheimer JT
FAU - Warden, K J
AU  - Warden KJ
FAU - Irby, R B
AU  - Irby RB
LA  - eng
GR  - HD-31644/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Carrier Proteins)
RN  - 0 (Plant Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (sterol carrier proteins)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - B7IN85G1HY (Dinoprost)
RN  - EC 1.1.- (3-Hydroxysteroid Dehydrogenases)
RN  - EC 1.14.15.6 (Cholesterol Side-Chain Cleavage Enzyme)
SB  - IM
MH  - 3-Hydroxysteroid Dehydrogenases/genetics
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Carrier Proteins/*genetics
MH  - Cholesterol Side-Chain Cleavage Enzyme/genetics
MH  - Dinoprost/*pharmacology
MH  - Female
MH  - Gene Expression/*drug effects
MH  - Molecular Sequence Data
MH  - Ovary/*metabolism
MH  - *Plant Proteins
MH  - Progesterone/blood
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
AID - 10.1210/endo.136.11.7588230 [doi]
PST - ppublish
SO  - Endocrinology. 1995 Nov;136(11):4963-72. doi: 10.1210/endo.136.11.7588230.