PMID- 7588400
OWN - NLM
STAT- MEDLINE
DCOM- 19951214
LR  - 20191023
IS  - 0743-5800 (Print)
IS  - 0743-5800 (Linking)
VI  - 21
IP  - 1-2
DP  - 1995 Feb-May
TI  - Ovine 11 beta-hydroxysteroid dehydrogenase: from gene to function.
PG  - 367-77
AB  - Two distinct isoforms of 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD) with 
      respect to enzymatic activity were identified in the ovine liver and kidney. 11 
      beta-HSD1 (the hepatic isoform) was reversible and NADP(H)-dependent. By 
      contrast, 11 beta-HSD2 (the renal isoform) was unidirectional and NAD-dependent. 
      Ovine placenta contained both forms of 11 beta-HSD activities. The cDNA encoding 
      ovine 11 beta-HSD1 was cloned, and used as a probe to study 11 beta-HSD1 gene 
      expression in fetal sheep during development. It was found that fetal and adult 
      liver was the major site of 11 beta-HSD1 biosynthesis, and that 11 beta-HSD1 gene 
      expression was regulated in a tissue-specific and developmentally programmed 
      manner. Two non-functional variants of 11 beta-HSD1 were also identified. In 
      addition, sheep kidney was unique in that both 11 beta-HSD1 mRNA and activity 
      were absent. Although the physiological significance of 11 beta-HSD in individual 
      fetal organs during development remains largely speculative, 11 beta-HSD in the 
      fetal pituitary may contribute, at least in part, to the proposed resetting of 
      cortisol negative feedback on pituitary ACTH during the last few days of 
      gestation. In the fetal liver, the action of 11 beta-HSD may lead to the 
      formation of cortisol which could act locally as well as systematically to 
      modulate developmental processes. Placental 11 beta-HSD may protect fetus from 
      exposure to the growth-inhibiting effects of maternal glucocorticoids.
FAU - Yang, K
AU  - Yang K
AD  - Lawson Research Institute, St. Joseph's Hospital, Department of Ob/Gyn, 
      University of Western Ontario, London, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - England
TA  - Endocr Res
JT  - Endocrine research
JID - 8408548
RN  - 0 (Isoenzymes)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenases
MH  - Animals
MH  - Gene Expression Regulation, Developmental/*physiology
MH  - Gene Expression Regulation, Enzymologic/*physiology
MH  - Hydroxysteroid Dehydrogenases/*genetics/physiology
MH  - Isoenzymes/*genetics/physiology
MH  - Kidney/*enzymology
MH  - Liver/*enzymology
MH  - Organ Specificity/genetics
MH  - Sheep
RF  - 49
EDAT- 1995/02/01 00:00
MHDA- 1995/02/01 00:01
CRDT- 1995/02/01 00:00
PHST- 1995/02/01 00:00 [pubmed]
PHST- 1995/02/01 00:01 [medline]
PHST- 1995/02/01 00:00 [entrez]
AID - 10.3109/07435809509030453 [doi]
PST - ppublish
SO  - Endocr Res. 1995 Feb-May;21(1-2):367-77. doi: 10.3109/07435809509030453.