PMID- 7589097
OWN - NLM
STAT- MEDLINE
DCOM- 19951212
LR  - 20120605
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 25
IP  - 10
DP  - 1995 Oct
TI  - Interleukin-7 activates p56lck and p59fyn, two tyrosine kinases associated with 
      the p90 interleukin-7 receptor in primary human T cells.
PG  - 2956-60
AB  - We have investigated signaling events associated with the cloned 90-kDa (p90) 
      interleukin-7 receptor (IL-7R) to determine whether changes in the signaling 
      pathways initiated by this molecule can explain the ability of T cells to 
      proliferate to IL-7 following activation. Using in vitro kinase assays we find 
      that the p90 IL-7R in both unstimulated and activated human T cells is physically 
      associated with two molecules with intrinsic kinase activity. Western blotting 
      analysis reveals these proteins to be the src kinase enzymes, p59fyn and p56lck. 
      Binding of human recombinant IL-7 to the p90 IL-7R results in increased activity 
      of both receptor-associated kinases in both resting and activated mature T cells. 
      Thus, the signaling pathways initiated via the p90 IL-7R-associated src kinases 
      are unlikely to be solely responsible for the proliferation of only activated T 
      cells in response to IL-7. Additional signals, which may derive from other 
      IL-7R-associated molecules such as the gamma c, are clearly required for 
      IL-7-driven proliferation of activated primary T cells.
FAU - Page, T H
AU  - Page TH
AD  - Kennedy Institute of Rheumatology, London, GB.
FAU - Lali, F V
AU  - Lali FV
FAU - Foxwell, B M
AU  - Foxwell BM
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Antigens, CD)
RN  - 0 (Interleukin-7)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-7)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.10.2 (FYN protein, human)
RN  - EC 2.7.10.2 (Lymphocyte Specific Protein Tyrosine Kinase p56(lck))
RN  - EC 2.7.10.2 (Proto-Oncogene Proteins c-fyn)
RN  - EC 2.7.10.2 (src-Family Kinases)
SB  - IM
MH  - Antigens, CD/*metabolism
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - Enzyme Activation/drug effects
MH  - Humans
MH  - Interleukin-7/*pharmacology
MH  - *Lymphocyte Activation
MH  - Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
MH  - Macromolecular Substances
MH  - Proto-Oncogene Proteins/*metabolism
MH  - Proto-Oncogene Proteins c-fyn
MH  - Receptors, Interleukin/*metabolism
MH  - Receptors, Interleukin-7
MH  - Recombinant Proteins/pharmacology
MH  - Signal Transduction/*physiology
MH  - T-Lymphocytes/*drug effects/enzymology
MH  - src-Family Kinases/*metabolism
EDAT- 1995/10/01 00:00
MHDA- 1995/10/01 00:01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 1995/10/01 00:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
AID - 10.1002/eji.1830251036 [doi]
PST - ppublish
SO  - Eur J Immunol. 1995 Oct;25(10):2956-60. doi: 10.1002/eji.1830251036.