PMID- 7589113
OWN - NLM
STAT- MEDLINE
DCOM- 19951128
LR  - 20041117
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 25
IP  - 9
DP  - 1995 Sep
TI  - CD34-positive early human thymocytes: T cell receptor and cytokine receptor gene 
      expression.
PG  - 2471-8
AB  - CD34, a stem cell marker, has been shown to be expressed on human CD3-CD4-CD8- 
      (triple-negative; TN) thymocytes. Phenotypic and functional analyses suggest the 
      following differentiation sequence: CD34+1-3-4-8(-)--> CD34+1+3-4 +/- 
      8(-)-->CD34-1+3-4+8(+/-)-->CD34-1++3-4+8+. In this report, we examined cytokine 
      receptor gene expression on these subsets by reverse transcription-polymerase 
      chain reaction analysis (RT-PCR). We were able to detect interleukin-7 receptor 
      (IL-7R), c-kit and IL-2R gamma in all CD34+ thymocyte subsets, consistent with 
      previous functional studies. We found IL-1R, granulocyte/macrophage 
      colony-stimulating factor receptor-alpha and IL-4R transcripts in CD3- and CD34+ 
      subsets. Secondly, we investigated T cell receptor (TCR)-delta and -beta gene 
      rearrangement and transcription in CD34+ thymocytes. Our results show that a 
      full-length TCR-delta transcript and the recombination activating genes RAG-1 and 
      RAG-2 mRNA were already expressed in the CD34+1- subset. Mature V beta-containing 
      TCR transcripts were also detected in the CD34+1+ subset, but not in the CD1- 
      fraction. Furthermore, PCR analysis of D-J beta gene rearrangements showed that > 
      or = 70% of CD34+1- cells are in a TCR beta germ-line configuration, although D-J 
      beta recombination had already started in this population.
FAU - Ktorza, S
AU  - Ktorza S
AD  - Laboratoire d'Immunologie Cellulaire et Tissulaire, CNRS URA 625, Hopital de la 
      Pitie-Salpetriere, Paris, France.
FAU - Sarun, S
AU  - Sarun S
FAU - Rieux-Laucat, F
AU  - Rieux-Laucat F
FAU - de Villartay, J P
AU  - de Villartay JP
FAU - Debre, P
AU  - Debre P
FAU - Schmitt, C
AU  - Schmitt C
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Antigens, CD34)
RN  - 0 (Receptors, Antigen, T-Cell)
RN  - 0 (Receptors, Cytokine)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Antigens, CD34/*biosynthesis
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Child
MH  - Child, Preschool
MH  - DNA/analysis
MH  - Gene Expression
MH  - Humans
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Receptors, Antigen, T-Cell/biosynthesis/genetics/*immunology
MH  - Receptors, Cytokine/biosynthesis/genetics/*immunology
MH  - Thymus Gland/*immunology/metabolism
EDAT- 1995/09/01 00:00
MHDA- 1995/09/01 00:01
CRDT- 1995/09/01 00:00
PHST- 1995/09/01 00:00 [pubmed]
PHST- 1995/09/01 00:01 [medline]
PHST- 1995/09/01 00:00 [entrez]
AID - 10.1002/eji.1830250910 [doi]
PST - ppublish
SO  - Eur J Immunol. 1995 Sep;25(9):2471-8. doi: 10.1002/eji.1830250910.