PMID- 7589325
OWN - NLM
STAT- MEDLINE
DCOM- 19951208
LR  - 20131121
IS  - 0014-4886 (Print)
IS  - 0014-4886 (Linking)
VI  - 135
IP  - 2
DP  - 1995 Oct
TI  - Protective role of nerve growth factor against excitatory amino acid injury 
      during neostriatal cholinergic neurons postnatal development.
PG  - 146-52
AB  - The interaction between excitatory amino acids (EAAs) and nerve growth factor 
      (NGF) levels were studied on neostriatal cholinergic neurons during postnatal 
      development. Striatal choline acetyltransferase (ChAT) activity and NGF levels 
      were determined 7 days following EAA injection in 7-, 15-, 21-, 30-, and 
      50-day-old rats. ChAT activity was decreased 7 days after kainate (KA), 
      quinolinate (QUIN), or quisqualate (QUIS) lesion. The reduction was most 
      pronounced in 30-day-old rats. KA injection produced the greatest decrease in 
      ChAT activity. Conversely, KA did not change NGF levels. QUIN and QUIS increased 
      NGF protein and these effects were maximal with lesions in 21-day-old rats. In 
      order to further characterize the effect of EAAs on NGF levels and ChAT activity, 
      the time-course of the lesion was studied. We used 30-day-old rats as the maximal 
      sensitivity of cholinergic neurons to EAAs was observed at this age. ChAT 
      activity decreased 2 days following QUIN or QUIS injection and 1 day after KA. 
      The EAA agonists also changed NGF levels. QUIN induced an increase in NGF levels 
      1 day after lesion. This effect was maintained to the last time point examined. 
      In contrast, KA and QUIS induced transient increases in NGF levels that were only 
      detected 2 and 4 days after injection, respectively. To study whether NGF is able 
      to regulate EAA excitotoxicity on striatal cholinergic neurons, we studied ChAT 
      activity 7 days after simultaneous injection of NGF plus QUIN, KA, or QUIS. 
      Intrastriatal injection of exogenous NGF was able to block the decrease in ChAT 
      activity observed following EAA injection alone.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Perez-Navarro, E
AU  - Perez-Navarro E
AD  - Departament de Biologia Celp4lar i Anatomia Patologica, Facultat de Medicina, 
      Universitat de Barcelona, Spain.
FAU - Alberch, J
AU  - Alberch J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Neurol
JT  - Experimental neurology
JID - 0370712
RN  - 0 (Excitatory Amino Acids)
RN  - 0 (Nerve Growth Factors)
RN  - F6F0HK1URN (Quinolinic Acid)
RN  - SIV03811UC (Kainic Acid)
SB  - IM
MH  - Animals
MH  - Animals, Newborn/*growth & development
MH  - Cholinergic Fibers/*drug effects
MH  - Excitatory Amino Acids/*pharmacology
MH  - Kainic Acid/pharmacology
MH  - Male
MH  - Neostriatum/*drug effects
MH  - Nerve Growth Factors/*pharmacology
MH  - Quinolinic Acid/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
EDAT- 1995/10/01 00:00
MHDA- 1995/10/01 00:01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 1995/10/01 00:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
AID - S0014-4886(85)71073-4 [pii]
AID - 10.1006/exnr.1995.1073 [doi]
PST - ppublish
SO  - Exp Neurol. 1995 Oct;135(2):146-52. doi: 10.1006/exnr.1995.1073.