PMID- 7589633
OWN - NLM
STAT- MEDLINE
DCOM- 19951207
LR  - 20190707
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 64
IP  - 5
DP  - 1995 Nov
TI  - Role of major histocompatibility complex class I expression and natural 
      killer-like T cells in the genetic control of endometriosis.
PG  - 909-16
AB  - OBJECTIVE: To evaluate whether the expression of human leukocyte antigen (HLA) 
      class I on eutopic and ectopic endometrial cells modify the susceptibility to 
      lysis mediated by lymphocytes. DESIGN: Evaluation of T lymphocyte cytotoxic 
      activity and HLA class I expression on endometrial cells. SETTING: Subjects were 
      recruited at laparoscopy. PATIENTS: Patients with endometriosis (n = 7). Healthy 
      women as controls (n = 10). MAIN OUTCOME MEASURES: Human leukocyte antigen class 
      I molecule analysis of endometrial cells was carried out by immunofluorescence 
      and flow cytometry. Phenotyping of T lymphocytes was performed to analyze T-cell 
      subsets. Cytotoxicity was performed to determine cytolytic activity against 
      endometrial cells. RESULTS: In vitro culture of endometrial cells down-regulates 
      the expression of HLA class I molecules and enhances the susceptibility to lysis 
      mediated by natural killer (NK)-like T lymphocytes. Cytolytic T-cell clones, 
      expressing the CD94 antigen, are inhibited by the HLA-B7 allele on endometrial 
      cells. Ectopic endometrial cells modulate the expression of HLA class I 
      molecules. CONCLUSIONS: The resistance to lysis of endometrial cells is related 
      to expression of surface HLA class I molecules, which send a negative signal for 
      lysis mediated by NK-like T lymphocytes. The HLA-B7 allele inhibits the cytotoxic 
      activity, suggesting that the growth of ectopic endometrial cells might be under 
      a genetic control.
FAU - Semino, C
AU  - Semino C
AD  - Servizio di Citometria, Istituto Nazionale per la Ricerca sul Cancro, Genova, 
      Italy.
FAU - Semino, A
AU  - Semino A
FAU - Pietra, G
AU  - Pietra G
FAU - Mingari, M C
AU  - Mingari MC
FAU - Barocci, S
AU  - Barocci S
FAU - Venturini, P L
AU  - Venturini PL
FAU - Ragni, N
AU  - Ragni N
FAU - Melioli, G
AU  - Melioli G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 0 (HLA-B7 Antigen)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Alleles
MH  - Cells, Cultured
MH  - Down-Regulation
MH  - Endometriosis/etiology/*genetics/*physiopathology
MH  - Endometrium/chemistry/metabolism/pathology
MH  - Female
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique, Indirect
MH  - Gene Expression Regulation
MH  - Genes, MHC Class I
MH  - HLA-B7 Antigen/analysis/genetics
MH  - Histocompatibility Antigens Class I/biosynthesis/genetics/*physiology
MH  - Humans
MH  - Interferon-gamma/pharmacology
MH  - Killer Cells, Natural/immunology/pathology/*physiology
MH  - Phenotype
MH  - T-Lymphocytes, Cytotoxic/pathology/physiology
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
AID - S0015-0282(16)57901-1 [pii]
AID - 10.1016/s0015-0282(16)57901-1 [doi]
PST - ppublish
SO  - Fertil Steril. 1995 Nov;64(5):909-16. doi: 10.1016/s0015-0282(16)57901-1.