PMID- 7589850
OWN - NLM
STAT- MEDLINE
DCOM- 19951228
LR  - 20190515
IS  - 0012-1797 (Print)
IS  - 0012-1797 (Linking)
VI  - 44
IP  - 12
DP  - 1995 Dec
TI  - Regulation of hexokinase II gene transcription and glucose phosphorylation by 
      catecholamines, cyclic AMP, and insulin.
PG  - 1426-32
AB  - The hexokinases, by converting glucose to glucose-6-phosphate, help maintain the 
      downhill gradient that results in movement of glucose into cells through the 
      facilitative glucose transporters. GLUT4 and hexokinase (HK) II are the major 
      transporter and hexokinase isoforms in skeletal muscle, heart, and adipose 
      tissue, wherein insulin promotes glucose utilization. To understand whether 
      hormones influence the contribution of phosphorylation to cellular glucose 
      utilization, we investigated the effects that catecholamines, cyclic AMP (cAMP), 
      and insulin have on HKII gene expression in cells representative of muscle (L6 
      cells) and brown (BFC-1B cells) and white (3T3-F442A cells) adipose tissues. 
      Isoproterenol or the cAMP analog 8-chlorophenylthio-cAMP selectively increase 
      HKII gene transcription in L6 cells, as does insulin (Printz RL, Koch S, Potter 
      LP, O'Doherty RM, Tiesinga JJ, Moritz S, Granner DK: Hexokinase II mRNA and gene 
      structure, regulation by insulin, and evolution. J Biol Chem 268:5209-5219, 
      1993), and cause a concentration- and time-dependent increase of HKII mRNA in 
      both muscle and fat cell lines without changing HKI mRNA. Isoproterenol and 
      insulin also increase the rate of synthesis of HKII protein and increase glucose 
      phosphorylation and glucose utilization in L6 cells.
FAU - Osawa, H
AU  - Osawa H
AD  - Department of Molecular Physiology and Biophysics, Vanderbilt University School 
      of Medicine, Nashville, Tennessee 37232, USA.
FAU - Printz, R L
AU  - Printz RL
FAU - Whitesell, R R
AU  - Whitesell RR
FAU - Granner, D K
AU  - Granner DK
LA  - eng
GR  - DK-35107/DK/NIDDK NIH HHS/United States
GR  - DK-42502/DK/NIDDK NIH HHS/United States
GR  - DK-46867/DK/NIDDK NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Diabetes
JT  - Diabetes
JID - 0372763
RN  - 0 (Catecholamines)
RN  - 0 (Insulin)
RN  - 0 (RNA, Messenger)
RN  - 0 (Thionucleotides)
RN  - 41941-66-6 (8-((4-chlorophenyl)thio)cyclic-3',5'-AMP)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - IY9XDZ35W2 (Glucose)
RN  - L628TT009W (Isoproterenol)
SB  - IM
MH  - 3T3 Cells
MH  - Adipose Tissue/enzymology
MH  - Adipose Tissue, Brown/enzymology
MH  - Animals
MH  - Catecholamines/*pharmacology
MH  - Cell Line
MH  - Cyclic AMP/analogs & derivatives/*pharmacology
MH  - Gene Expression Regulation/*drug effects
MH  - Glucose/*metabolism
MH  - Hexokinase/*genetics
MH  - Insulin/*pharmacology
MH  - Isoproterenol/pharmacology
MH  - Mice
MH  - Muscles/enzymology
MH  - Phosphorylation
MH  - RNA, Messenger/metabolism
MH  - Thionucleotides/pharmacology
MH  - Transcription, Genetic
EDAT- 1995/12/01 00:00
MHDA- 1995/12/01 00:01
CRDT- 1995/12/01 00:00
PHST- 1995/12/01 00:00 [pubmed]
PHST- 1995/12/01 00:01 [medline]
PHST- 1995/12/01 00:00 [entrez]
AID - 10.2337/diab.44.12.1426 [doi]
PST - ppublish
SO  - Diabetes. 1995 Dec;44(12):1426-32. doi: 10.2337/diab.44.12.1426.