PMID- 7590268
OWN - NLM
STAT- MEDLINE
DCOM- 19951130
LR  - 20211203
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 163
IP  - 2
DP  - 1995 Oct 3
TI  - The Drosophila melanogaster homolog of the mammalian MAPK-activated protein 
      kinase-2 (MAPKAPK-2) lacks a proline-rich N-terminus.
PG  - 209-14
AB  - Recently, a mammalian kinase cascade was discovered that is triggered by stress 
      and heat shock, and leads to the stimulation of mitogen-activated protein kinase 
      (MAPK)-activated protein kinase-2 (MAPKAPK-2). Surprisingly, this process turns 
      out to be independent of the classical MAPK. The stress-induced activation of 
      MAPKAPK-2, in turn, results in the phosphorylation of small heat-shock proteins 
      (Hsp). We have isolated a Drosophila melanogaster (Dm) cDNA encoding a 
      polypeptide that has extensive sequence similarity to the mammalian MAPKAPK-2. As 
      in mammalian MAPKAPK-2, the Dm MAPKAPK-2 possesses a MAPK phosphorylation site 
      and a nuclear targeting sequence located C-terminal to the catalytic domain. 
      However, in contrast to its mammalian counterpart, it lacks the Pro-rich 
      N-terminal region proposed to form Src-homology domain 3 (SH3) binding domains. A 
      2.4-kb MAPKAPK-2 message is expressed throughout development, while two shorter 
      transcripts of 2.3 and 1.8 kb appear to be specifically expressed in the 
      germline. The 1.8-kb transcript results from the usage of an atypical 
      germline-specific polyadenylation signal (AATATA) located early within the 3' 
      untranslated region. Dm MAPKAPK-2 is located at cytological position 5D in the Dm 
      genome.
FAU - Larochelle, S
AU  - Larochelle S
AD  - Department of Biology, McGill University, Montreal, Quebec, Canada.
FAU - Suter, B
AU  - Suter B
LA  - eng
SI  - GENBANK/U20757
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Heat-Shock Proteins)
RN  - 9DLQ4CIU6V (Proline)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 2)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - Drosophila melanogaster/*genetics
MH  - Heat-Shock Proteins/*genetics
MH  - MAP Kinase Kinase 2
MH  - *Mitogen-Activated Protein Kinase Kinases
MH  - Molecular Sequence Data
MH  - Proline/genetics
MH  - Protein Serine-Threonine Kinases/chemistry/*genetics
MH  - Protein-Tyrosine Kinases/chemistry/*genetics
MH  - Sequence Alignment
MH  - Sequence Analysis
EDAT- 1995/10/03 00:00
MHDA- 1995/10/03 00:01
CRDT- 1995/10/03 00:00
PHST- 1995/10/03 00:00 [pubmed]
PHST- 1995/10/03 00:01 [medline]
PHST- 1995/10/03 00:00 [entrez]
AID - 0378-1119(95)00279-F [pii]
AID - 10.1016/0378-1119(95)00279-f [doi]
PST - ppublish
SO  - Gene. 1995 Oct 3;163(2):209-14. doi: 10.1016/0378-1119(95)00279-f.