PMID- 7590286
OWN - NLM
STAT- MEDLINE
DCOM- 19951130
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 163
IP  - 2
DP  - 1995 Oct 3
TI  - Human peptidylglycine alpha-amidating monooxygenase transcripts derived by 
      alternative mRNA splicing of an unreported exon.
PG  - 307-11
AB  - We are characterizing the alternatively spliced human peptidylglycine 
      alpha-amidating monooxygenase (hPAM)-encoding mRNA transcripts expressed by human 
      cells. Reverse transcription coupled to the polymerase chain reaction (RT-PCR) 
      has been used to identify four alternatively spliced variants that differ in the 
      region joining the two catalytic domains. Two of the transcripts represent 
      previously reported splice variants differentiated by the presence (hPAM-A) or 
      absence (hPAM-B) of a 321-nucleotide (nt) linker (optional exon A) which in the 
      rat produce functionally distinct enzymes. Different mRNAs represent two splice 
      variants, hPAM-C and hPAM-D, that show the presence of an exon unreported for PAM 
      in any other species. This new exon, designated exon C, is 54 nt in length, 
      encodes an 18-amino-acid (aa) peptide containing a conserved dibasic aa 
      endoproteolytic processing motif, and is located 3' of exon A in human genomic 
      DNA. We propose that cell-specific regulation of mRNA splicing would provide a 
      mechanism for control of prohormone activation by these variants of the PAM 
      enzyme.
FAU - Vos, M D
AU  - Vos MD
AD  - Intervention Section, National Cancer Institute Division of Cancer Prevention and 
      Control, Rockville, MD 20850-3300, USA.
FAU - Jones, J E
AU  - Jones JE
FAU - Treston, A M
AU  - Treston AM
LA  - eng
SI  - GENBANK/U20089
SI  - GENBANK/U20090
PT  - Journal Article
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Multienzyme Complexes)
RN  - 0 (RNA, Messenger)
RN  - EC 1.- (Mixed Function Oxygenases)
RN  - EC 1.14.17.3 (peptidylglycine monooxygenase)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Exons/genetics
MH  - Humans
MH  - Mixed Function Oxygenases/biosynthesis/*genetics
MH  - Molecular Sequence Data
MH  - *Multienzyme Complexes
MH  - RNA, Messenger/genetics
MH  - Rats
EDAT- 1995/10/03 00:00
MHDA- 1995/10/03 00:01
CRDT- 1995/10/03 00:00
PHST- 1995/10/03 00:00 [pubmed]
PHST- 1995/10/03 00:01 [medline]
PHST- 1995/10/03 00:00 [entrez]
AID - 0378-1119(95)00364-C [pii]
AID - 10.1016/0378-1119(95)00364-c [doi]
PST - ppublish
SO  - Gene. 1995 Oct 3;163(2):307-11. doi: 10.1016/0378-1119(95)00364-c.