PMID- 7590353
OWN - NLM
STAT- MEDLINE
DCOM- 19951226
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 164
IP  - 2
DP  - 1995 Oct 27
TI  - Restriction map of a 35-kb HLA fragment constructed by nested deletion 'drop-out' 
      mapping.
PG  - 335-9
AB  - An efficient method for generating detailed restriction maps of large cloned DNA 
      segments is demonstrated. The mapping strategy entails comparing restriction 
      fragments from a parent clone and from nested deletion derivatives of that clone. 
      In a set of deletion plasmids of decreasing size, an individual fragment will be 
      lost, or 'drop-out', according to its position in the cloned fragment. In this 
      demonstration, nested deletions were generated in both directions in a 35-kb DNA 
      segment from the human leukocyte antigen (HLA) region by intramolecular 
      transposition of an engineered gamma delta (Tn1000) element present in a special 
      'deletion factory' cloning vector [Wang et al., Proc. Natl. Acad. Sci. USA 90 
      (1993) 7874-7878]. Fifteen plasmids with deletions extending in one direction and 
      eleven plasmids with deletions extending in the opposite direction were digested 
      singly by each of four restriction enzymes. A total of 36 cleavage sites were 
      mapped in the 35-kb HLA fragment. This drop-out approach using nested deletions 
      provides a simple and efficient means of mapping restriction sites, genes and 
      other features of interest in cosmid-sized cloned DNA segments or DNAs.
FAU - LaBanca, F
AU  - LaBanca F
AD  - Department of Molecular and Cell Biology, University of Connecticut, Storrs 
      06269-2131, USA.
FAU - Krishnan, B R
AU  - Krishnan BR
FAU - Chaplin, D D
AU  - Chaplin DD
FAU - Berg, D E
AU  - Berg DE
FAU - Berg, C M
AU  - Berg CM
LA  - eng
GR  - AI34580/AI/NIAID NIH HHS/United States
GR  - DK48029/DK/NIDDK NIH HHS/United States
GR  - HG00820/HG/NHGRI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (DNA Transposable Elements)
RN  - 0 (HLA Antigens)
RN  - EC 3.1.21.- (DNA Restriction Enzymes)
SB  - IM
MH  - Animals
MH  - Cloning, Molecular
MH  - DNA Restriction Enzymes/metabolism
MH  - DNA Transposable Elements
MH  - Genetic Techniques
MH  - HLA Antigens/*genetics
MH  - Hominidae/*genetics
MH  - Humans
MH  - *Major Histocompatibility Complex
MH  - Mutagenesis
MH  - *Restriction Mapping
MH  - Sequence Deletion
EDAT- 1995/10/27 00:00
MHDA- 1995/10/27 00:01
CRDT- 1995/10/27 00:00
PHST- 1995/10/27 00:00 [pubmed]
PHST- 1995/10/27 00:01 [medline]
PHST- 1995/10/27 00:00 [entrez]
AID - 037811199500462F [pii]
AID - 10.1016/0378-1119(95)00462-f [doi]
PST - ppublish
SO  - Gene. 1995 Oct 27;164(2):335-9. doi: 10.1016/0378-1119(95)00462-f.