PMID- 7591030
OWN - NLM
STAT- MEDLINE
DCOM- 19951128
LR  - 20121115
IS  - 0894-1491 (Print)
IS  - 0894-1491 (Linking)
VI  - 14
IP  - 3
DP  - 1995 Jul
TI  - Biochemical subtypes of oligodendrocyte in the anterior medullary velum of the 
      rat as revealed by the monoclonal antibody Rip.
PG  - 185-97
AB  - Oligodendrocytes were studied in the anterior medullary velum (AMV) of the rat 
      using the monoclonal antibody Rip, an oligodendrocyte marker of unknown function. 
      Confocal microscopic imaging of double immunofluorescent labelling with 
      antibodies to Rip and carbonic anhydrase II (CAII) revealed two biochemically and 
      morphologically distinct populations of oligodendrocyte which were either 
      Rip+CAII+ or Rip+CAII-. Double immunofluorescent labelling with Rip and myelin 
      basic protein (MBP) or glial fibrillary acidic protein (GFAP) provided direct 
      evidence that Rip-labelled cells were phenotypically oligodendrocytes and 
      confirmed that Rip did not recognise astrocytes. Oligodendrocytes which were 
      Rip+CAII+ supported numerous myelin sheaths for small diameter axons, whilst 
      Rip+CAII- oligodendrocytes supported fewer myelin sheaths for large diameter 
      axons. Morphologically, Rip+CAII+ oligodendrocytes corresponded to types I or II 
      of classical nomenclature, whilst Rip+CAII- oligodendrocytes corresponded to 
      types III and IV. The results demonstrated a biochemical difference between 
      oligodendrocytes which myelinated small and large diameter fibres.
FAU - Butt, A M
AU  - Butt AM
AD  - Division of Physiology, U.M.D.S., Guy's Hospital, London, United Kingdom.
FAU - Ibrahim, M
AU  - Ibrahim M
FAU - Ruge, F M
AU  - Ruge FM
FAU - Berry, M
AU  - Berry M
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Glia
JT  - Glia
JID - 8806785
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Myelin Basic Protein)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Carbonic Anhydrases/analysis
MH  - Fluorescent Antibody Technique
MH  - Glial Fibrillary Acidic Protein/analysis
MH  - Male
MH  - Medulla Oblongata/*cytology
MH  - Microscopy, Confocal
MH  - Myelin Basic Protein/analysis
MH  - Oligodendroglia/*chemistry/immunology
MH  - Phenotype
MH  - Rats
MH  - Rats, Wistar
EDAT- 1995/07/01 00:00
MHDA- 1995/07/01 00:01
CRDT- 1995/07/01 00:00
PHST- 1995/07/01 00:00 [pubmed]
PHST- 1995/07/01 00:01 [medline]
PHST- 1995/07/01 00:00 [entrez]
AID - 10.1002/glia.440140304 [doi]
PST - ppublish
SO  - Glia. 1995 Jul;14(3):185-97. doi: 10.1002/glia.440140304.