PMID- 7591119
OWN - NLM
STAT- MEDLINE
DCOM- 19951228
LR  - 20220310
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 63
IP  - 12
DP  - 1995 Dec
TI  - Cytokine mRNA expression profiles in rats infected with the intestinal nematode 
      Nippostrongylus brasiliensis.
PG  - 4653-60
AB  - Although the immune responses to intestinal nematode infection have been well 
      studied and have been shown to be strongly driven by Th2-associated cytokines in 
      mice, such information has been limited with respect to rats. We investigated 
      changes in levels of the mRNAs encoding interleukin-2 (IL-2), IL-3, IL-4, IL-5, 
      IL-10, and gamma interferon in the mesenteric lymph nodes of rats infected with 
      Nippostrongylus brasiliensis by reverse transcription-PCR in comparison with 
      immunoglobulin E (IgE)/IgG2a antibody, eosinophil, basophil, and mucosal mast 
      cell responses. In the two rat strains used, Brown Norway and Fischer-344, which 
      show different responses to allergens, serum IgE increased to much higher levels 
      in the former than in the latter 2 weeks after infection. Intestinal mastocytosis 
      was observed much earlier and more intensely in Brown Norway rats than in 
      Fischer-344 rats, but the degrees of peripheral eosinophilia and basophilia did 
      not differ between the two strains. In both strains, IL-3, IL-4, and IL-5 mRNA 
      expression increased and peaked around 7 to 14 days after infection, while 
      expression of IL-2, IL-10, and gamma interferon mRNAs did not change notably 
      throughout the experimental period. The highest IL-4 mRNA expression was observed 
      slightly earlier in Brown Norway than in Fischer-344 rats, but levels of IL-3 and 
      IL-5 mRNAs peaked synchronously in both strains. The amounts of mRNAs encoding 
      these three cytokines were always higher in Brown Norway than in Fischer-344 
      rats. It is suggested that in rats, Th2 or Th2-like cells are also induced after 
      nematode infection, and IgE elevation is mainly related to increased IL-4 gene 
      expression.
FAU - Matsuda, S
AU  - Matsuda S
AD  - Department of Medical Zoology, Kyoto Prefectural University of Medicine, Japan.
FAU - Uchikawa, R
AU  - Uchikawa R
FAU - Yamada, M
AU  - Yamada M
FAU - Arizono, N
AU  - Arizono N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cytokines/*biosynthesis/genetics
MH  - Interferon-gamma/genetics
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nippostrongylus/*immunology
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/*biosynthesis
MH  - Rats
MH  - Rats, Inbred BN
MH  - Rats, Inbred F344
MH  - Species Specificity
MH  - Strongylida Infections/*immunology
MH  - T-Lymphocytes, Helper-Inducer/immunology
PMC - PMC173668
EDAT- 1995/12/01 00:00
MHDA- 1995/12/01 00:01
PMCR- 1995/12/01
CRDT- 1995/12/01 00:00
PHST- 1995/12/01 00:00 [pubmed]
PHST- 1995/12/01 00:01 [medline]
PHST- 1995/12/01 00:00 [entrez]
PHST- 1995/12/01 00:00 [pmc-release]
AID - 10.1128/iai.63.12.4653-4660.1995 [doi]
PST - ppublish
SO  - Infect Immun. 1995 Dec;63(12):4653-60. doi: 10.1128/iai.63.12.4653-4660.1995.