PMID- 7591288
OWN - NLM
STAT- MEDLINE
DCOM- 19951228
LR  - 20190708
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 63
IP  - 5
DP  - 1995 Nov 27
TI  - Fibroblast growth factor 3 is tumorigenic for mouse mammary cells orthotopically 
      implanted in nude mice.
PG  - 702-9
AB  - Fibroblast growth factor-3 (Fgf-3) is involved in mouse mammary tumorigenesis 
      since the Fgf-3 gene is a main target for mouse mammary tumor virus (MMTV) 
      insertional activation. Its action has been correlated with the appearance of 
      pregnancy-dependent tumors. We describe here the effects on normal mouse mammary 
      EF43 cells of the short Fgf-3 protein form which enters the secretory pathway. 
      The genes, Fgf-3 AUG or Fgf-4 for comparison, were introduced in the mammary 
      cells by means of retroviral vectors. Fgf-3 expression did not modify EF43 cell 
      morphology, had no effect on growth in soft agar nor on the inhibitory action 
      exerted on cell growth by TGF-beta 1; however, it allowed the cells to grow under 
      low serum conditions in the absence of insulin and EGF. The Fgf-3-expressing 
      cells were not tumorigenic in nude mice when injected s.c., but tumors developed 
      when the cells were implanted in the mammary gland. The tumors appeared after 
      some latency; they had a slow growth phase followed by a phase of increased 
      growth rate. An identical tumoral growth pattern was observed in ovariectomized 
      nude mice. These results show that the secreted Fgf-3 form can initiate 
      tumorigenesis and that the induced tumors are hormone-independent. The 
      mammary-gland environment, however, is required for the EF43 cells to grow and 
      differentiate. During that process, which resembles natural cell growth during 
      mammary-gland development at pregnancy, the cells could pass through a stage 
      which is specifically sensitive to Fgf-3.
FAU - Hajitou, A
AU  - Hajitou A
AD  - Laboratory of Fundamental Virology, University of Liege, Belgium.
FAU - Calberg-Bacq, C M
AU  - Calberg-Bacq CM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (Fgf3 protein, mouse)
RN  - 0 (Fibroblast Growth Factor 3)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 62031-54-3 (Fibroblast Growth Factors)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Cell Division/drug effects
MH  - Cell Transformation, Neoplastic/*genetics
MH  - Cell Transplantation
MH  - Dogs
MH  - Female
MH  - Fibroblast Growth Factor 3
MH  - Fibroblast Growth Factors/*genetics/physiology
MH  - Gene Expression
MH  - Mammary Glands, Animal/cytology
MH  - Mammary Neoplasms, Experimental/*genetics
MH  - Mice
MH  - Mice, Nude
MH  - Neoplasms, Hormone-Dependent/*genetics
MH  - Proto-Oncogene Proteins/*genetics/physiology
MH  - Transfection
EDAT- 1995/11/27 00:00
MHDA- 1995/11/27 00:01
CRDT- 1995/11/27 00:00
PHST- 1995/11/27 00:00 [pubmed]
PHST- 1995/11/27 00:01 [medline]
PHST- 1995/11/27 00:00 [entrez]
AID - 10.1002/ijc.2910630516 [doi]
PST - ppublish
SO  - Int J Cancer. 1995 Nov 27;63(5):702-9. doi: 10.1002/ijc.2910630516.