PMID- 7593211
OWN - NLM
STAT- MEDLINE
DCOM- 19951205
LR  - 20041117
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 165
IP  - 2
DP  - 1995 Nov
TI  - Hepatocyte growth factor/scatter factor expressed in follicular papilla cells 
      stimulates human hair growth in vitro.
PG  - 333-8
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) is a multifunctional polypeptide 
      which acts as mitogen, motogen, or morphogen. In this study, we examined the 
      effect of HGF/SF on human hair growth using organ and cell culture systems. 
      HGF/SF was found to stimulate hair length and DNA synthesis in hair follicles at 
      increasing concentrations up to 10 ng/ml (P < 0.05 and P < 0.01, respectively). 
      HGF/SF stimulated [3H]thymidine incorporation by hair bulb-derived keratinocytes 
      with the strongest response at 30 ng/ml of HGF/SF (P < 0.05). Cultured follicular 
      papilla cells secreted HGF/SF, measured by an enzyme-linked immunoassay, in 
      response to interleukin 1-alpha (IL1-alpha, 10 ng/ml), tumor necrosis 
      factor-alpha (TNF-alpha, 10 ng/ml), or tetradecanoylphorbolacetate (100 nM) at 
      levels ranging from 0.2 to 0.3 ng/mg protein/48 h. HGF/SF mRNA expressions, 
      measured by the reverse transcription-polymerase chain reaction, were detected in 
      follicular papilla cells, and were also stimulated by the three reagents. 
      Transforming growth factor-beta (10 ng/ml) suppressed both protein and mRNA 
      levels. These results suggest that hair follicle elongation induced by HGF/SF in 
      organ culture occurs partly due to the mitogenic activity of HGF/SF expressed in 
      follicular papilla cells on hair bulb-derived keratinocytes.
FAU - Shimaoka, S
AU  - Shimaoka S
AD  - Department of Dermatology, Juntendo University, School of Medicine, Tokyo, Japan.
FAU - Tsuboi, R
AU  - Tsuboi R
FAU - Jindo, T
AU  - Jindo T
FAU - Imai, R
AU  - Imai R
FAU - Takamori, K
AU  - Takamori K
FAU - Rubin, J S
AU  - Rubin JS
FAU - Ogawa, H
AU  - Ogawa H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Molecular Probes)
RN  - 0 (RNA, Messenger)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Culture Techniques
MH  - Hair/cytology/*drug effects/*growth & development
MH  - Hair Follicle/cytology/*metabolism
MH  - Hepatocyte Growth Factor/genetics/*physiology
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Keratinocytes/drug effects
MH  - Molecular Probes/genetics
MH  - Molecular Sequence Data
MH  - Organ Culture Techniques
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/metabolism
MH  - Transcription, Genetic
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
AID - 10.1002/jcp.1041650214 [doi]
PST - ppublish
SO  - J Cell Physiol. 1995 Nov;165(2):333-8. doi: 10.1002/jcp.1041650214.