PMID- 7593318
OWN - NLM
STAT- MEDLINE
DCOM- 19951215
LR  - 20220215
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 108 ( Pt 8)
DP  - 1995 Aug
TI  - Induction and regulation of connexin26 by glucagon in primary cultures of adult 
      rat hepatocytes.
PG  - 2771-80
AB  - In the adult rat hepatocyte, the gap junction proteins consist of a major 
      component, connexin32 (Cx32) and a minor component, connexin26 (Cx26). Although 
      we recently reported our success in inducing and maintaining Cx32 in adult rat 
      hepatocytes cultured in serum-free L-15 medium supplemented with epidermal growth 
      factor and 2% dimethyl sulfoxide, it was very difficult to induce Cx26 in the 
      primary hepatocytes. In the present study, we found that the addition of 10(-7) M 
      glucagon into the culture medium could dramatically induce Cx26 mRNA and protein. 
      Although the expression of Cx32 mRNA was also influenced by glucagon, the 
      increase of the expression was small. Immunocytochemically, Cx26-positive spots 
      were observed between most adjacent cells and were co-localized with the 
      Cx32-positive spots. We also examined whether 0.5 mM dibutyl cyclic AMP could 
      induce expression of Cx26 in the cells. The effect of dexamethasone on the 
      expression of Cx26 mRNA compared to that of Cx32 mRNA was examined. For the 
      induction and maintenance of Cx26 mRNA, more than 10(-7) M dexamethasone was 
      necessary in this culture. These results suggest that expression of Cx26 in 
      hepatocytes may be regulated by the concentrations of glucagon and glucocorticoid 
      hormones.
FAU - Kojima, T
AU  - Kojima T
AD  - Department of Pathology, Sapporo Medical University School of Medicine, Japan.
FAU - Mitaka, T
AU  - Mitaka T
FAU - Shibata, Y
AU  - Shibata Y
FAU - Mochizuki, Y
AU  - Mochizuki Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Connexins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Gjb2 protein, rat)
RN  - 0 (Isoquinolines)
RN  - 0 (RNA, Messenger)
RN  - 127120-53-0 (Connexin 26)
RN  - 63X7MBT2LQ (Bucladesine)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 9007-92-5 (Glucagon)
RN  - 9654F8OVKE (lucifer yellow)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Bucladesine/pharmacology
MH  - Cells, Cultured
MH  - Connexin 26
MH  - Connexins/analysis/*biosynthesis
MH  - Dexamethasone/pharmacology
MH  - Fluorescent Antibody Technique
MH  - Fluorescent Dyes
MH  - Gene Expression/*drug effects
MH  - Gene Expression Regulation/drug effects
MH  - Glucagon/*pharmacology
MH  - Immunohistochemistry
MH  - Isoquinolines
MH  - Kinetics
MH  - Liver/cytology/drug effects/*metabolism
MH  - Male
MH  - Microscopy, Confocal
MH  - RNA, Messenger/analysis/biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
EDAT- 1995/08/01 00:00
MHDA- 1995/08/01 00:01
CRDT- 1995/08/01 00:00
PHST- 1995/08/01 00:00 [pubmed]
PHST- 1995/08/01 00:01 [medline]
PHST- 1995/08/01 00:00 [entrez]
AID - 10.1242/jcs.108.8.2771 [doi]
PST - ppublish
SO  - J Cell Sci. 1995 Aug;108 ( Pt 8):2771-80. doi: 10.1242/jcs.108.8.2771.