PMID- 7594486
OWN - NLM
STAT- MEDLINE
DCOM- 19951218
LR  - 20101118
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 155
IP  - 10
DP  - 1995 Nov 15
TI  - High level monocyte chemoattractant protein-1 expression in transgenic mice 
      increases their susceptibility to intracellular pathogens.
PG  - 4838-43
AB  - We have constructed transgenic mice in which the mouse mammary tumor virus long 
      terminal repeat controls the expression of murine monocyte chemoattractant 
      protein-1 (MCP-1). Several independently derived lines of transgenic mice 
      constitutively expressed MCP-1 protein in a variety of organs. Protein extracts 
      from these organs had substantial in vitro monocyte chemoattractant activity that 
      was neutralized by an anti-MCP-1 Ab, indicating that transgenic MCP-1 protein is 
      biologically active. However, no transgenic mouse at any age displayed monocyte 
      infiltrates in MCP-1-expressing organs. Two transgenic lines had circulating 
      MCP-1 levels of 13 to 26 ng/ml, which is a concentration sufficient to induce 
      maximal monocyte chemotaxis in vitro. These transgenic lines showed a 1 to 1.5 
      log greater sensitivity to infection with Listeria monocytogenes and 
      Mycobacterium tuberculosis. A third transgenic line had lower serum levels of 
      MCP-1 and was resistant to L. monocytogenes. The results suggest that this 
      transgenic model is one of monocyte nonresponsiveness to locally produced MCP-1 
      due to either receptor desensitization or neutralization of a chemoattractant 
      gradient by high systemic concentrations of MCP-1. Regardless of the mechanism, 
      the data indicate that constitutively high levels of MCP-1 expression do not 
      induce monocytic infiltrates, and that MCP-1 is involved in the host response to 
      intracellular pathogens.
FAU - Rutledge, B J
AU  - Rutledge BJ
AD  - Department of Medicine, Dana-Farber Cancer Institute, Harvard Medical School, 
      Boston, MA 02115, USA.
FAU - Rayburn, H
AU  - Rayburn H
FAU - Rosenberg, R
AU  - Rosenberg R
FAU - North, R J
AU  - North RJ
FAU - Gladue, R P
AU  - Gladue RP
FAU - Corless, C L
AU  - Corless CL
FAU - Rollins, B J
AU  - Rollins BJ
LA  - eng
GR  - CA53091/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Animals
MH  - Cell Movement
MH  - Chemokine CCL2/*biosynthesis/genetics/immunology
MH  - Chemotaxis
MH  - *Immune Tolerance
MH  - Listeria monocytogenes/*immunology/pathogenicity
MH  - Listeriosis/*immunology/pathology
MH  - Mice
MH  - Mice, Transgenic
MH  - Monocytes/*immunology
MH  - Mycobacterium tuberculosis/*immunology/pathogenicity
MH  - Tuberculosis/*immunology/pathology
EDAT- 1995/11/15 00:00
MHDA- 1995/11/15 00:01
CRDT- 1995/11/15 00:00
PHST- 1995/11/15 00:00 [pubmed]
PHST- 1995/11/15 00:01 [medline]
PHST- 1995/11/15 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1995 Nov 15;155(10):4838-43.