PMID- 7594520
OWN - NLM
STAT- MEDLINE
DCOM- 19951228
LR  - 20101118
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 155
IP  - 11
DP  - 1995 Dec 1
TI  - Differences in the avidity of TCR interactions with a superantigenic ligand 
      affect negative selection but do not allow positive selection.
PG  - 5115-23
AB  - The products of the sag genes of the exogenous mouse mammary tumor virus (MMTV) 
      genome and of endogenous Mtv integrants have been demonstrated to affect the T 
      cell repertoire in mice by causing the deletion of T cells expressing receptors 
      encoded by particular V beta gene segments. Since these deletions affect large 
      populations of T cells with receptors of heterogeneous specificity, they serve as 
      an important model for the study of T cell development in normal mice. Using 
      several C3H/HeN-based strains that express different MMTV(C3H) transgenes, we 
      demonstrate here that the stage of development at which T cell deletion occurs is 
      determined by the level of ligand expression. Although at low levels of ligand 
      expression in the thymus some signs of activation were observed in immature 
      thymocytes, we were unable to detect a level of Sag expression that led to net 
      positive selection. Moreover, we detected a level of Sag-transgene expression 
      that did not cause negative selection in the thymus; no signs of positive 
      selection were observed either. Inclusion of the env gene in the construct, 
      earlier shown to markedly potentiate stimulation by Sag in mixed lymphocyte 
      reactions, also markedly increased the ability of Sag to drive negative 
      selection. These data are interpreted as showing that marked quantitative 
      differences in expression of superantigens does not reveal a level at which only 
      positive selection occurs. This, in turn, suggests that positive selection will 
      occur on ligands distinct from those that drive clonal deletion.
FAU - Chervonsky, A V
AU  - Chervonsky AV
AD  - Section of Immunobiology, Yale University School of Medicine, New Haven, CT 
      06520, USA.
FAU - Golovkina, T V
AU  - Golovkina TV
FAU - Ross, S R
AU  - Ross SR
FAU - Janeway, C A Jr
AU  - Janeway CA Jr
LA  - eng
GR  - AI-14579/AI/NIAID NIH HHS/United States
GR  - CA45954/CA/NCI NIH HHS/United States
GR  - CA52646/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
RN  - 0 (Antigens, Viral)
RN  - 0 (CD69 antigen)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
RN  - 0 (Superantigens)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Aging/immunology
MH  - Animals
MH  - Antigens, CD/biosynthesis
MH  - Antigens, Differentiation, T-Lymphocyte/biosynthesis
MH  - Antigens, Viral/*immunology
MH  - Base Sequence
MH  - Cell Differentiation/immunology
MH  - Cells, Cultured
MH  - Clonal Deletion/immunology
MH  - Lectins, C-Type
MH  - Mammary Tumor Virus, Mouse/*immunology
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, Inbred CBA
MH  - Mice, Transgenic
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Receptors, Antigen, T-Cell, alpha-beta/immunology
MH  - Superantigens/*immunology
MH  - T-Lymphocytes/cytology/*immunology
MH  - Thymus Gland/cytology/immunology
MH  - Viral Envelope Proteins/biosynthesis/immunology
EDAT- 1995/12/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1995/12/01 00:00
PHST- 1995/12/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1995/12/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1995 Dec 1;155(11):5115-23.