PMID- 7594524
OWN - NLM
STAT- MEDLINE
DCOM- 19951228
LR  - 20071114
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 155
IP  - 11
DP  - 1995 Dec 1
TI  - Mapping the Ig superantigen-binding site of HIV-1 gp120.
PG  - 5151-9
AB  - The envelope glycoprotein, gp120, of HIV-1 has recently been identified as a 
      member of the new family of Ig superantigens (Ig-SAg). This classification is 
      based on the selective binding of gp120 to an unusually high proportion of 
      endogenous, nonimmune Ig, and the selective activation of nonimmune B cells by 
      gp120 in vitro. Many, if not all of the nonimmune Ig that bind to gp120 are 
      members of the VH3 Ig gene family. The aim of this study was to determine the 
      epitope on gp120 that was responsible for its Ig-SAg binding activity. To do 
      this, we utilized a panel of 30 peptides derived from gp160 in a 
      competition-binding assay. For five Igs that were tested, as well as for 
      polyclonal serum IgM, two overlapping peptides (each 20 amino acids in length) 
      were identified that were potent inhibitors of gp120 binding. Similarly, the 10 
      amino acid overlap region of these two peptides had inhibitory activity. Thus, 
      this decamer sequence represented the optimal Ig-SAg epitope or mimotope. The 
      amino acid residue at position 1 of the decamer, and to a lesser extent at 
      position 10, was critical for peptide binding. In addition to this decamer 
      peptide, other peptides that shared modest sequence homology were also 
      selectively inhibitory for specific Ig samples. These findings provide the first 
      definition of an Ig-SAg ligand at the peptide level and will facilitate further 
      structural and biologic characterization of this new class of pathogenic Ags.
FAU - Goodglick, L
AU  - Goodglick L
AD  - Department of Pathology and Laboratory Medicine, Jonsson Comprehensive Cancer 
      Center, UCLA School of Medicine 90095, USA.
FAU - Zevit, N
AU  - Zevit N
FAU - Neshat, M S
AU  - Neshat MS
FAU - Braun, J
AU  - Braun J
LA  - eng
GR  - CA12800/CA/NCI NIH HHS/United States
GR  - CA49308/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (HIV Envelope Protein gp120)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Peptides)
RN  - 0 (Superantigens)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antibodies, Monoclonal/immunology
MH  - *Epitope Mapping
MH  - HIV Envelope Protein gp120/*metabolism
MH  - HIV-1/*immunology
MH  - Humans
MH  - Immunoglobulin M/immunology/metabolism
MH  - Molecular Sequence Data
MH  - Peptides/immunology
MH  - Superantigens/*metabolism
EDAT- 1995/12/01 00:00
MHDA- 1995/12/01 00:01
CRDT- 1995/12/01 00:00
PHST- 1995/12/01 00:00 [pubmed]
PHST- 1995/12/01 00:01 [medline]
PHST- 1995/12/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1995 Dec 1;155(11):5151-9.