PMID- 7594969
OWN - NLM
STAT- MEDLINE
DCOM- 19951214
LR  - 20190909
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 68
IP  - 4
DP  - 1995 Oct
TI  - Fluorescence in situ hybridization detection of chromosomal aberrations in human 
      lymphocytes: applicability to biological dosimetry.
PG  - 429-35
AB  - Human lymphocytes in G0 have been irradiated with X-ray doses from 0 to 4.0 Gy. 
      Metaphase chromosomes 2, 3 and 5 and all centromeres were painted using 
      fluorescence in situ hybridization (FISH) probe libraries. Dicentrics, centric 
      rings and acentrics in the whole genome as well as translocations involving the 
      painted chromosomes were recorded. The translocations were subdivided as complete 
      or incomplete. Interstitial insertions and inversions were also noted. The 
      observations were also recorded according to the Protocol for Aberration 
      Identification and Nomenclature Terminology (PAINT) system of scoring. Given that 
      the painted chromosomes comprise 20.4% of the genome it was found that the yield 
      of bicoloured dicentrics was consistent with the yield of dicentrics in the whole 
      genome. The yield of radiation-induced translocations was not significantly 
      higher than that of bicoloured dicentrics. Of the translocations, 60% were 
      complete and it was concluded that the majority of dicentrics and translocations 
      are complete exchanges. Chromosome 5 took part in exchanges marginally more 
      commonly than its length suggests, but it is not known if this is a property of 
      the chromosome or whether it is a donor-dependent observation. The PAINT system 
      of recording rearrangements was examined and the suggested numerical 
      interpretation of this nomenclature was considered to be unsuitable for use in 
      the estimation of dose for cases of accidental overexposure.
FAU - Finnon, P
AU  - Finnon P
AD  - National Radiological Protection Board, Chilton, Didcot, UK.
FAU - Lloyd, D C
AU  - Lloyd DC
FAU - Edwards, A A
AU  - Edwards AA
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
SB  - IM
MH  - Adult
MH  - *Chromosome Aberrations
MH  - Dose-Response Relationship, Radiation
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Lymphocytes/*ultrastructure
MH  - Male
MH  - Translocation, Genetic
EDAT- 1995/10/01 00:00
MHDA- 1995/10/01 00:01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 1995/10/01 00:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
AID - KYQ21JLV1J0GFTNT [pii]
AID - 10.1080/09553009514551391 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 1995 Oct;68(4):429-35. doi: 10.1080/09553009514551391.