PMID- 7595035
OWN - NLM
STAT- MEDLINE
DCOM- 19951221
LR  - 20181130
IS  - 0022-2143 (Print)
IS  - 0022-2143 (Linking)
VI  - 126
IP  - 5
DP  - 1995 Nov
TI  - Antibodies to neutrophil cytoplasmic antigens induce monocyte chemoattractant 
      protein-1 secretion from human monocytes.
PG  - 495-502
AB  - Antibodies to neutrophil cytoplasmic antigens (ANCA) have been found in the serum 
      samples of patients with a number of vasculitides (e.g., Wegener's 
      granulomatosis, small vessel vasculitis, and idiopathic necrotizing and cresentic 
      glomerulonephritis). Although detection of ANCA in serum samples has proven to be 
      useful diagnostically and in selected activity of disease monitoring situations, 
      the pathogenetic role of ANCA in vasculitis remains ill-defined. We sought to 
      determine whether purified ANCA promotes the secretion of monocyte 
      chemoattractant protein-1 (MCP-1) from isolated human peripheral blood monocytes. 
      P (perinuclear)- and C (cytoplasmic)- ANCA were purified from the serum samples 
      of patients with either Wegener's granulomatosis, small vessel vasculitis, or 
      idiopathic necrotizing and cresentic glomerulonephritis. Human peripheral blood 
      monocytes from healthy subjects were incubated with either C-ANCA immunoglobulin 
      G (IgG), P-ANCA IgG, or nonspecific IgG, and the conditioned media were analyzed 
      for MCP-1 activity. A monocyte chemotaxis assay was utilized to functionally 
      quantify secreted chemotactic activity. Secretion of monocyte chemotactic 
      activity was found to be antibody concentration-dependent and time-dependent, 
      with maximal chemotaxis measured in media collected 24 hours after the addition 
      of either C- or P-ANCA IgG. A specific antibody directed against human MCP-1 
      largely inhibited monocyte chemotaxis, indicating that MCP-1 is the predominant 
      monocyte chemotactic mediator present in the conditioned medium. An MCP-1 
      enzyme-linked immunosorbent assay further supported the conclusion that P- and 
      C-ANCA IgG can trigger MCP-1 secretion by monocytes. These data indicate that 
      incubation of monocytes with ANCA promotes the dose-dependent release of the 
      chemotactic beta-chemokine MCP-1.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Casselman, B L
AU  - Casselman BL
AD  - Department of Pathology, University of Michigan Medical School, Ann Arbor 
      48109-0602, USA.
FAU - Kilgore, K S
AU  - Kilgore KS
FAU - Miller, B F
AU  - Miller BF
FAU - Warren, J S
AU  - Warren JS
LA  - eng
GR  - 5T 32-HL07517/HL/NHLBI NIH HHS/United States
GR  - HL48287/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Lab Clin Med
JT  - The Journal of laboratory and clinical medicine
JID - 0375375
RN  - 0 (Antibodies, Antineutrophil Cytoplasmic)
RN  - 0 (Autoantibodies)
RN  - 0 (Biomarkers)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Antibodies, Antineutrophil Cytoplasmic
MH  - Autoantibodies/isolation & purification/*pharmacology
MH  - Biomarkers
MH  - Chemokine CCL2/*metabolism
MH  - Chemotaxis, Leukocyte
MH  - Dose-Response Relationship, Drug
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Granulomatosis with Polyangiitis/complications
MH  - Humans
MH  - Immunoglobulin G/pharmacology
MH  - Monocytes/drug effects/*metabolism
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
PST - ppublish
SO  - J Lab Clin Med. 1995 Nov;126(5):495-502.