PMID- 7595301 OWN - NLM STAT- MEDLINE DCOM- 19951207 LR - 20061115 IS - 0022-1198 (Print) IS - 0022-1198 (Linking) VI - 40 IP - 4 DP - 1995 Jul TI - Typing of DNA HLA-DQ alpha alleles extracted from human nail material using polymerase chain reaction. PG - 634-6 AB - The deoxyribonucleic acid (DNA) typing of human Leukocyte Antigen (HLA) DQ alpha from human fingernails is described. HLA-DQ alpha genotypes can be accurately determined from clipped fingernails. We have typed 26 nails accurately, while one did not give any type since that one sample did not amplify due to the low quantity of DNA. The cut off limit for the digested material to be amplified is approximately 9 mgs of nail material. FAU - Tahir, M A AU - Tahir MA AD - Indianapolis-Marion County Forensic Services Agency, IN, USA. FAU - Watson, N AU - Watson N LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - J Forensic Sci JT - Journal of forensic sciences JID - 0375370 RN - 0 (HLA-DQ Antigens) RN - 9007-49-2 (DNA) SB - IM MH - *Alleles MH - DNA/*analysis MH - HLA-DQ Antigens/*genetics MH - Humans MH - Nails/*chemistry MH - Polymerase Chain Reaction MH - Reproducibility of Results EDAT- 1995/07/01 00:00 MHDA- 1995/07/01 00:01 CRDT- 1995/07/01 00:00 PHST- 1995/07/01 00:00 [pubmed] PHST- 1995/07/01 00:01 [medline] PHST- 1995/07/01 00:00 [entrez] PST - ppublish SO - J Forensic Sci. 1995 Jul;40(4):634-6.