PMID- 7595528
OWN - NLM
STAT- MEDLINE
DCOM- 19951204
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 65
IP  - 5
DP  - 1995 Nov
TI  - beta-Amyloid1-40 increases expression of beta-amyloid precursor protein in 
      neuronal hybrid cells.
PG  - 2373-6
AB  - Studies of cell injury and death in Alzheimer's disease have suggested a 
      prominent role for beta-amyloid peptide (beta-AP), a 40-43-amino-acid peptide 
      derived from a larger membrane glycoprotein, beta-amyloid precursor protein 
      (beta-APP). Previous experiments have demonstrated that beta-AP induces 
      cytotoxicity in a neuronal hybrid cell line (MES 23.5) in vitro. Here, we 
      demonstrate that beta-APP mRNA content is increased 3.5-fold in 24 h after 
      treatment with beta-AP1-40. Accompanying beta-AP1-40-induced cell injury, levels 
      of cell-associated beta-APP and a C-terminal intermediate fragment are increased 
      up to 15-fold, and levels of secreted forms of beta-APP and 12- and 4-kDa 
      fragments are also increased. Application of beta-APP antisense 
      oligodeoxynucleotide reduces both cytotoxicity and beta-APP expression. 
      6-Hydroxydopamine application or glucose deprivation causes extensive cell 
      damage, but they do not increase beta-APP expression. These results suggest a 
      selective positive feedback mechanism whereby beta-AP may induce cytotoxicity and 
      increase levels of potentially neurotrophic as well as amyloidogenic fragments of 
      beta-APP with the net consequence of further neuronal damage.
FAU - Le, W
AU  - Le W
AD  - Department of Neurology, Baylor College of Medicine, Houston, TX 77030, USA.
FAU - Xie, W J
AU  - Xie WJ
FAU - Nyormoi, O
AU  - Nyormoi O
FAU - Ho, B K
AU  - Ho BK
FAU - Smith, R G
AU  - Smith RG
FAU - Appel, S H
AU  - Appel SH
LA  - eng
GR  - 3 P50 AG08664-0551/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Molecular Probes)
RN  - 0 (Peptide Fragments)
RN  - 0 (amyloid beta-protein (1-40))
SB  - IM
MH  - Amyloid beta-Peptides/*pharmacology
MH  - Amyloid beta-Protein Precursor/*metabolism
MH  - Base Sequence
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Hybrid Cells/*metabolism
MH  - Molecular Probes/genetics
MH  - Molecular Sequence Data
MH  - Neurons/cytology/drug effects/*metabolism
MH  - Peptide Fragments/*pharmacology
MH  - Polymerase Chain Reaction
MH  - Transcription, Genetic
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
AID - 10.1046/j.1471-4159.1995.65052373.x [doi]
PST - ppublish
SO  - J Neurochem. 1995 Nov;65(5):2373-6. doi: 10.1046/j.1471-4159.1995.65052373.x.