PMID- 7595957
OWN - NLM
STAT- MEDLINE
DCOM- 19950728
LR  - 20151119
IS  - 0362-2525 (Print)
IS  - 0022-2887 (Linking)
VI  - 224
IP  - 3
DP  - 1995 Jun
TI  - Lectin histochemical identification of carbohydrate moieties in opossum 
      chemosensory systems during development, with special emphasis on VVA-identified 
      subdivisions in the accessory olfactory bulb.
PG  - 331-49
AB  - Lectins, sugar-binding molecules of nonimmune origin, were used in this study to 
      describe the development of the main olfactory and vomeronasal systems in the 
      Brazilian gray short-tailed opossum, Monodelphis domestica. A battery of seven 
      lectins of the N-acetylgalactosamine/galactose-binding group was used. Of the 
      seven lectins, only two, Vicia villosa agglutinin (VVA) and Griffonia 
      (Bandeiraea) simplicifolia lectin I-isolectin B4 (GS I-B4), were specific to the 
      vomeronasal system. The other five lectins recognized carbohydrates in both 
      chemosensory systems, although the binding was more intense in the accessory 
      olfactory system. Furthermore, whereas six of the lectins stained the adult 
      opossum accessory olfactory bulb (AOB) homogeneously, the VVA lectin 
      distinguished two regions of the AOB. Similar to the expression of olfactory 
      marker protein (OMP) (Shnayder et al. [1993] Neuroreport 5:193-196), the rostral 
      half of the AOB stained much darker with VVA than the caudal half, and the onset 
      of the restricted pattern of staining at age 45 days also coincided. We conclude 
      that 1) GS I-B4 and VVA recognize cell surface carbohydrate moieties specific to 
      the vomeronasal, but not to the main olfactory, system, and 2) the carbohydrate 
      moiety that is recognized by the VVA lectin, presumably terminal 
      N-acetyl-galactosamine, is both temporally and spatially restricted in the 
      opossum AOB. These results are discussed in the framework of other known 
      spatially restricted molecules of the two major nasal chemosensory systems.
FAU - Shapiro, L S
AU  - Shapiro LS
AD  - Program in Neural and Behavioral Sciences, Health Science Center at Brooklyn, 
      State University of New York 11203, USA.
FAU - EE, P L
AU  - EE PL
FAU - Halpern, M
AU  - Halpern M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Morphol
JT  - Journal of morphology
JID - 0406125
RN  - 0 (Biomarkers)
RN  - 0 (Carbohydrates)
RN  - 0 (Lectins)
RN  - 0 (Plant Lectins)
RN  - 0 (Vicia lectins)
RN  - KM15WK8O5T (Acetylgalactosamine)
RN  - X2RN3Q8DNE (Galactose)
SB  - IM
MH  - Acetylgalactosamine/isolation & purification
MH  - Animals
MH  - Biomarkers
MH  - Carbohydrates/*isolation & purification
MH  - Galactose/isolation & purification
MH  - Histocytochemistry
MH  - *Lectins
MH  - Olfactory Bulb/anatomy & histology/*chemistry
MH  - Opossums/*anatomy & histology/growth & development
MH  - *Plant Lectins
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
AID - 10.1002/jmor.1052240307 [doi]
PST - ppublish
SO  - J Morphol. 1995 Jun;224(3):331-49. doi: 10.1002/jmor.1052240307.