PMID- 7596415
OWN - NLM
STAT- MEDLINE
DCOM- 19950803
LR  - 20111117
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 375
IP  - 6534
DP  - 1995 Jun 29
TI  - Mediation by HLA-DM of dissociation of peptides from HLA-DR.
PG  - 802-6
AB  - Human leukocyte antigen (HLA)-DM is an unconventional major histocompatibility 
      complex (MHC) class II heterodimer that is important for B-cell-mediated antigen 
      processing and presentation to MHC class II-restricted T cells. HLA-DM is encoded 
      by two genes, DMA and DMB, which map to the MHC class II region, and shares some 
      homology with MHC class I and class II proteins. Here we define the biochemical 
      role of HLA-DM. Recombinant soluble HLA-DM heterodimers have been purified from 
      culture supernatants of insect cell transformants. At pH 5.0, they induce the 
      dissociation of a subset of peptides bound to HLA-DR, including a nested set of 
      class-II-associated invariant chain peptides (CLIP). This process liberates 
      HLA-DR and leads to the enhanced binding of exogenous peptides.
FAU - Sloan, V S
AU  - Sloan VS
AD  - Department of Autoimmune Disease Research, Merck Research Laboratories, Rahway, 
      New Jersey 07065, USA.
FAU - Cameron, P
AU  - Cameron P
FAU - Porter, G
AU  - Porter G
FAU - Gammon, M
AU  - Gammon M
FAU - Amaya, M
AU  - Amaya M
FAU - Mellins, E
AU  - Mellins E
FAU - Zaller, D M
AU  - Zaller DM
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (Antigens, Differentiation, B-Lymphocyte)
RN  - 0 (H2-M antigens)
RN  - 0 (HLA-D Antigens)
RN  - 0 (HLA-DM antigens)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Oligopeptides)
RN  - 0 (invariant chain)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Antigens, Differentiation, B-Lymphocyte
MH  - B-Lymphocytes/*immunology
MH  - Cell Line
MH  - Drosophila
MH  - HLA-D Antigens/*physiology
MH  - HLA-DR Antigens/chemistry/*metabolism
MH  - Histocompatibility Antigens Class II/metabolism
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Molecular Sequence Data
MH  - Oligopeptides/*metabolism
MH  - Protein Conformation
MH  - Transformation, Genetic
EDAT- 1995/06/29 00:00
MHDA- 1995/06/29 00:01
CRDT- 1995/06/29 00:00
PHST- 1995/06/29 00:00 [pubmed]
PHST- 1995/06/29 00:01 [medline]
PHST- 1995/06/29 00:00 [entrez]
AID - 10.1038/375802a0 [doi]
PST - ppublish
SO  - Nature. 1995 Jun 29;375(6534):802-6. doi: 10.1038/375802a0.