PMID- 7598092
OWN - NLM
STAT- MEDLINE
DCOM- 19950731
LR  - 20161123
IS  - 0192-415X (Print)
IS  - 0192-415X (Linking)
VI  - 23
IP  - 1
DP  - 1995
TI  - Preventive effect of dai-saiko-to (da-chai-hu-tang) extract on disrupted hepatic 
      active oxygen metabolism in rats with carbon tetrachloride-induced liver injury.
PG  - 53-64
AB  - In order to clarify the preventive action of Dai-Saiko-to (Da-Chai-Hu-Tang) 
      extract (TJ-8) on the progression of acute liver injury in rats intoxicated with 
      carbon tetrachloride (CCl4), we examined the effect of post-oral TJ-8 
      administration on hepatic active oxygen metabolism following the progression of 
      this liver damage. When TJ-8 (1.0 g/kg body weight) was administered orally to 
      male Wistar rats aged five weeks 2 hrs after i.p. injection of CCl4 (1.0 ml/kg 
      body weight), an apparent liver injury occurred. Significant prevention against 
      the progression of liver injury was found at 24 hrs after injection, judging from 
      the activities of serum transaminases, indexes of liver cell damage. Liver 
      cytosolic superoxide dismutase (SOD) activity decreased 2 and 24 hrs after CCl4 
      injection, while liver cytosolic catalase and glutathione reductase (GSSG-R) 
      activities decreased 24 hrs after the injection. At 2 and 24 hrs after CCl4 
      treatment, liver cytosolic Se-containing glutathione peroxidase (GSH-px) activity 
      did not change and liver cytosolic glucose-6-phosphate dehydrogenase (G-6-PDH) 
      activity increased. Post-oral TJ-8 administration significantly ameliorated 
      decreases in liver SOD, catalase, and GSSG-R activities at 24 hrs after CCl4 
      injection, but did not affect liver Se-GSH-px and increased liver G-6-PDH 
      activities at 24 hrs after the injection. Although increased liver lipid peroxide 
      level and decreased liver reduced glutathione and ascorbic acid levels were 
      observed 2 and 24 hrs after CCl4 injection, post-oral TJ-8 administration 
      significantly prevented these changes found at 24 hrs after injection. These 
      results indicate that post-oral TJ-8 administration can prevent the progression 
      of acute liver injury in CCl4-injected rats by inhibiting enhanced lipid 
      peroxidation and by improving disrupted active oxygen metabolism in the injured 
      liver.
FAU - Ohta, Y
AU  - Ohta Y
AD  - Department of Biochemistry, School of Medicine, Fujita Health University, Japan.
FAU - Sasaki, E
AU  - Sasaki E
FAU - Nishida, K
AU  - Nishida K
FAU - Kobayashi, T
AU  - Kobayashi T
FAU - Nagata, M
AU  - Nagata M
FAU - Ishiguro, I
AU  - Ishiguro I
LA  - eng
PT  - Journal Article
PL  - Singapore
TA  - Am J Chin Med
JT  - The American journal of Chinese medicine
JID - 7901431
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Lipid Peroxides)
RN  - 30KYC7MIAI (Aspartic Acid)
RN  - CL2T97X0V0 (Carbon Tetrachloride)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 2.6.1.2 (Alanine Transaminase)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Administration, Oral
MH  - Alanine Transaminase/drug effects
MH  - Animals
MH  - Aspartic Acid/metabolism
MH  - Carbon Tetrachloride
MH  - Chemical and Drug Induced Liver Injury
MH  - Drugs, Chinese Herbal/*therapeutic use
MH  - Glucosephosphate Dehydrogenase/drug effects
MH  - Lipid Peroxides/metabolism
MH  - Liver Diseases/*drug therapy/metabolism
MH  - Male
MH  - Oxygen/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Superoxide Dismutase/drug effects
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - S0192415X95000080 [pii]
AID - 10.1142/S0192415X95000080 [doi]
PST - ppublish
SO  - Am J Chin Med. 1995;23(1):53-64. doi: 10.1142/S0192415X95000080.