PMID- 7598693
OWN - NLM
STAT- MEDLINE
DCOM- 19950801
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 211
IP  - 3
DP  - 1995 Jun 26
TI  - G protein mutations in tumours of the pituitary, parathyroid and endocrine 
      pancreas.
PG  - 1063-70
AB  - A subset of growth-hormone (GH) producing pituitary adenomas harbour mutations at 
      residues Arg201 and Gln227 of the alpha subunit of the stimulatory G protein (Gs 
      alpha). One such mutation has been reported in a GH-producing tumour from a 
      patient with multiple endocrine neoplasia type 1 (MEN 1) although mutations have 
      not been reported in other tumours associated with MEN 1. We used PCR-induced 
      restriction site analysis to screen for these mutations in 80 tumours of the 
      pituitary, parathyroid and endocrine pancreas. Arg201 mutations were detected in 
      1 non-functioning and 4 GH-producing pituitary tumours. No mutations were found 
      in any of the endocrine tumours tested at Arg179 of the Gi2 alpha subunit, a 
      homologous residue to Arg201 of Gs alpha. Our results indicate oncogenesis in the 
      majority of pituitary and parathyroid tumours is independent of mutations of Gs 
      and Gi2 alpha.
FAU - Boothroyd, C V
AU  - Boothroyd CV
AD  - Queensland Cancer Fund Research Unit, Queensland Institute of Medical Research, 
      Herston, Australia.
FAU - Grimmond, S M
AU  - Grimmond SM
FAU - Cameron, D P
AU  - Cameron DP
FAU - Hayward, N K
AU  - Hayward NK
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 9002-72-6 (Growth Hormone)
RN  - EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Base Sequence
MH  - Deoxyribonucleases, Type II Site-Specific/metabolism
MH  - GTP-Binding Proteins/*genetics
MH  - Growth Hormone/metabolism
MH  - Humans
MH  - Islets of Langerhans/*pathology
MH  - Molecular Sequence Data
MH  - Multiple Endocrine Neoplasia Type 1/genetics
MH  - *Mutation
MH  - Pancreatic Neoplasms/*genetics
MH  - Parathyroid Neoplasms/*genetics
MH  - Pituitary Neoplasms/*genetics
MH  - Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
EDAT- 1995/06/26 00:00
MHDA- 1995/06/26 00:01
CRDT- 1995/06/26 00:00
PHST- 1995/06/26 00:00 [pubmed]
PHST- 1995/06/26 00:01 [medline]
PHST- 1995/06/26 00:00 [entrez]
AID - S0006291X85719195 [pii]
AID - 10.1006/bbrc.1995.1919 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1995 Jun 26;211(3):1063-70. doi: 
      10.1006/bbrc.1995.1919.