PMID- 7598795
OWN - NLM
STAT- MEDLINE
DCOM- 19950809
LR  - 20180215
IS  - 1015-2008 (Print)
IS  - 1015-2008 (Linking)
VI  - 62
IP  - 5-6
DP  - 1994
TI  - Differential adherence of the human monocyte subsets 27E10 and RM3/1 to cytokine- 
      or glucocorticoid-treated endothelial cells.
PG  - 262-8
AB  - Monocyte-endothelial interactions are of particular importance in the regulation 
      of inflammation. The aim of the present study was to investigate the adhesion of 
      functional different monocyte subsets to human umbilical vein endothelial cells 
      treated with various cytokines or a glucocorticoid. The adherence of monocyte 
      subset 27E10, which is associated with inflammatory processes, increased after 
      endothelial activation with interleukin-1 beta (IL-1 beta), interferon-gamma (IFN 
      gamma), tumor necrosis factor-alpha (TNF alpha), and the glucocorticoid 
      prednylidene (Pred). The adherence at IFN gamma-treated endothelial cells was 
      strong after a coculture duration of 10 min with a slight increase up to 60 min. 
      The peak value after TNF alpha stimulation was reached after 15 min, thereafter 
      quickly decreasing. IL-1 and Pred treatment caused a maximal adherence between 15 
      and 30 min followed by a slow decrease. TNF alpha and particularly interleukin-6 
      (IL-6) enhanced the endothelial adhesion of the monocyte subtype RM3/1, which is 
      associated with the downregulation of inflammation. The maximal adherence was 
      found after 15 and 30 min of coculture, respectively. The results show that, 
      through modulation of the adhesive properties of endothelial cells, cytokines and 
      glucocorticoids affect the adherence of monocyte subsets differently. They also 
      suggest that IL-6 plays a role in the downregulation of acute inflammation.
FAU - Hauptmann, S
AU  - Hauptmann S
AD  - Institute of Pathology, Technical University Aachen (RWTH), Germany.
FAU - Bernauer, J
AU  - Bernauer J
FAU - Zwadlo-Klarwasser, G
AU  - Zwadlo-Klarwasser G
FAU - Klosterhalfen, B
AU  - Klosterhalfen B
FAU - Kirkpatrick, C J
AU  - Kirkpatrick CJ
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Pathobiology
JT  - Pathobiology : journal of immunopathology, molecular and cellular biology
JID - 9007504
RN  - 0 (Cytokines)
RN  - 0 (Glucocorticoids)
RN  - 0 (Interleukins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9PHQ9Y1OLM (Prednisolone)
RN  - IF8PQP966U (prednylidene)
SB  - IM
MH  - Cell Adhesion/*physiology
MH  - Cytokines/*pharmacology
MH  - Endothelium, Vascular/*cytology/physiology
MH  - Glucocorticoids/*pharmacology
MH  - Humans
MH  - Interferon-gamma/pharmacology
MH  - Interleukins/pharmacology
MH  - Monocytes/*physiology/ultrastructure
MH  - Prednisolone/*analogs & derivatives/pharmacology
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Umbilical Veins/cytology
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1159/000163919 [doi]
PST - ppublish
SO  - Pathobiology. 1994;62(5-6):262-8. doi: 10.1159/000163919.