PMID- 7599027
OWN - NLM
STAT- MEDLINE
DCOM- 19950808
LR  - 20190904
IS  - 0168-1605 (Print)
IS  - 0168-1605 (Linking)
VI  - 25
IP  - 1
DP  - 1995 Mar
TI  - Evaluation of diluents and media for enumerating Zygosaccharomyces rouxii in 
      blueberry syrup.
PG  - 11-8
AB  - Combinations of diluents and enumeration media were evaluated for their efficacy 
      in enumerating Zygosaccharomyces rouxii in blueberry syrup (aw 0.818, 0.870 and 
      0.921). Diluents consisted of deionized water containing 0.1% peptone, 20%, 30%, 
      40% or 50% glucose, 50% glucose plus 0.05% Tween 80, and 12%, 18%, 26% or 35% 
      glycerol, all calculated on a w/w basis. Enumeration media were dichloran rose 
      bengal chloramphenicol (DRBC) agar, tryptone glucose yeast extract (TGY) agar, 
      dichloran 18% glycerol (DG18) agar, plate count agar containing 52% sucrose 
      (PCA52S) and malt extract yeast extract 50% glucose (MY50G) agar. Two test 
      strains of Z. rouxii grown in blueberry syrup for 7 or 14 days responded 
      similarly to diluent/enumeration medium combinations. The use of 0.1% peptone 
      diluent or DRBC agar in combination with other enumeration media or diluents was 
      inferior for recovering Z. rouxii. As the aw of the blueberry syrup decreased, 
      the sensitivity of Z. rouxii to diluent containing decreased concentrations of 
      glucose increased. The use of glycerol as a solute in diluent did not result in a 
      similar trend, indicating that the protective effect of glycerol against osmotic 
      stress to Z. rouxii cells is greater than that of glucose at high aw and that 
      cells adapted to low aw in blueberry syrup are more sensitive to high aw in 
      diluents. The addition of 0.05% Tween 80 to 50% glucose diluent did not influence 
      performance. Overall, diluents containing 50% glucose (aw 0.898), 18% glycerol 
      (aw 0.956) or 26% glycerol (0.934), in combination with TGY agar (aw 0.982), 
      resulted in the highest recovery of Z. rouxii from blueberry syrup at aw 0.818 to 
      0.921.
FAU - Hernandez, P
AU  - Hernandez P
AD  - Facultad de Farmacia, Universidad Central de Venezuela, Caracas.
FAU - Beuchat, L R
AU  - Beuchat LR
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Int J Food Microbiol
JT  - International journal of food microbiology
JID - 8412849
RN  - 0 (Culture Media)
RN  - 0 (Peptones)
RN  - IY9XDZ35W2 (Glucose)
RN  - PDC6A3C0OX (Glycerol)
SB  - IM
MH  - Colony Count, Microbial
MH  - *Culture Media
MH  - *Food Microbiology
MH  - Fruit/*microbiology
MH  - Glucose/metabolism
MH  - Glycerol/metabolism
MH  - Peptones/metabolism
MH  - Saccharomycetales/growth & development/*isolation & purification
EDAT- 1995/03/01 00:00
MHDA- 1995/03/01 00:01
CRDT- 1995/03/01 00:00
PHST- 1995/03/01 00:00 [pubmed]
PHST- 1995/03/01 00:01 [medline]
PHST- 1995/03/01 00:00 [entrez]
AID - 0168-1605(94)00041-4 [pii]
AID - 10.1016/0168-1605(94)00041-4 [doi]
PST - ppublish
SO  - Int J Food Microbiol. 1995 Mar;25(1):11-8. doi: 10.1016/0168-1605(94)00041-4.