PMID- 7599864
OWN - NLM
STAT- MEDLINE
DCOM- 19950810
LR  - 20171116
IS  - 1073-449X (Print)
IS  - 1073-449X (Linking)
VI  - 152
IP  - 1
DP  - 1995 Jul
TI  - Adoptive transfer of allergic airway responses with sensitized lymphocytes in BN 
      rats.
PG  - 64-70
AB  - To evaluate the role of lymphocytes in the pathogenesis of allergic 
      bronchoconstriction, we investigated whether allergic airway responses are 
      adoptively transferred by antigen-primed lymphocytes in Brown Norway (BN) rats. 
      Animals were actively sensitized to ovalbumin (OA) or sham sensitized, and 14 d 
      later mononuclear cells (MNCs) were isolated from intrathoracic lymph nodes, 
      passed through a nylon wool column, and transferred to naive syngeneic rats. 
      Recipients were challenged with aerosolized OA or bovine serum albumin (BSA) (5% 
      wt/vol) and analyzed for changes in lung resistance (RL), airway responsiveness 
      to inhaled methacholine (MCh), and bronchoalveolar lavage (BAL) cells. Recipients 
      of MNCs from sensitized rats responded to OA inhalation and exhibited sustained 
      increases in RL throughout the 8-h observation period, but without usual early 
      airway responses. Recipients of sham-sensitized MNCs or BSA-challenged recipients 
      failed to respond to antigen challenge. At 32 h after OA exposure, airway 
      responsiveness to MCh was increased in four of seven rats that had received 
      sensitized MNCs (p = 0.035). BAL eosinophils increased at 32 h in the recipients 
      of both sensitized and sham-sensitized MNCs. However, eosinophil numbers in BAL 
      were inversely correlated with airway responsiveness in the recipients of 
      sensitized MNCs (r = -0.788, p = 0.036). OA-specific immunoglobulin E (IgE) was 
      undetectable by enzyme-linked immunosorbent assay (ELISA) or passive cutaneous 
      anaphylaxis (PCA) in recipient rats following adoptive transfer. In conclusion, 
      allergic late airway responses (LAR) and cholinergic airway hyperresponsiveness, 
      but not antigen-specific IgE and early responses, were adoptively transferred by 
      antigen-primed lymphocytes in BN rats.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Watanabe, A
AU  - Watanabe A
AD  - Meakins-Christie Laboratories, McGill University, Royal Victoria Hospital, 
      Montreal, Quebec, Canada.
FAU - Rossi, P
AU  - Rossi P
FAU - Renzi, P M
AU  - Renzi PM
FAU - Xu, L J
AU  - Xu LJ
FAU - Guttmann, R D
AU  - Guttmann RD
FAU - Martin, J G
AU  - Martin JG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Respir Crit Care Med
JT  - American journal of respiratory and critical care medicine
JID - 9421642
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Bronchial Hyperreactivity/*immunology/physiopathology
MH  - Bronchial Provocation Tests
MH  - Bronchoalveolar Lavage Fluid/cytology
MH  - Eosinophils/immunology
MH  - Immunoglobulin E/immunology
MH  - *Immunotherapy, Adoptive
MH  - Male
MH  - Ovalbumin/immunology
MH  - Passive Cutaneous Anaphylaxis/immunology
MH  - Rats
MH  - Rats, Inbred BN
MH  - Rats, Sprague-Dawley
MH  - Respiratory Hypersensitivity/*immunology/physiopathology
MH  - Serum Albumin, Bovine/immunology
MH  - T-Lymphocytes/*immunology
EDAT- 1995/07/01 00:00
MHDA- 1995/07/01 00:01
CRDT- 1995/07/01 00:00
PHST- 1995/07/01 00:00 [pubmed]
PHST- 1995/07/01 00:01 [medline]
PHST- 1995/07/01 00:00 [entrez]
AID - 10.1164/ajrccm.152.1.7599864 [doi]
PST - ppublish
SO  - Am J Respir Crit Care Med. 1995 Jul;152(1):64-70. doi: 
      10.1164/ajrccm.152.1.7599864.