PMID- 7600900
OWN - NLM
STAT- MEDLINE
DCOM- 19950804
LR  - 20071114
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 20
IP  - 1
DP  - 1995 May 1
TI  - Karyotypic heterogeneity and its relation to labeling index in interphase breast 
      tumor cells.
PG  - 62-73
AB  - We have used fluorescence in situ hybridization (FISH) with chromosome-specific 
      probes and immunofluorescent detection of in vivo bromodeoxyuridine (BrdUrd) 
      incorporation to evaluate simultaneously numerical chromosome aberrations and 
      proliferative activity of breast cancers. The number of distinct hybridization 
      domains specific for repetitive pericentromeric sequences on chromosomes 1, 7, 
      11, 15, 17, and X was used as an indicator of copy number of these chromosomes in 
      interphase tumor cells from 23 human breast cancers. Every tumor analyzed showed 
      a heterogeneous distribution of copy number for at least one chromosome type. The 
      copy number distribution for different chromosomes within a tumor frequently 
      showed differing patterns. Major cell populations showing monosomy were 
      relatively rare, occurring only in five cases for chromosome 17, once for 
      chromosome 1, and once for chromosome 15. Flow cytometric analysis of DNA ploidy 
      correlated well with FISH analysis, although flow cytometry failed to detect 
      aneuploidy when only a few chromosomes were affected. To determine whether cell 
      populations with different chromosomal copy numbers have identical proliferation 
      characteristics in vivo, BrdUrd incorporation and centromeric copy number were 
      detected simultaneously. Comparison of the chromosome copy number distribution in 
      BrdUrd-positive cells vs. the distribution of the entire cell population showed 
      different distributions in seven of the 20 cases analyzed. This study 
      demonstrates the common occurrence of chromosome copy number heterogeneity and 
      suggests that a cell phenotype (proliferation) may be associated with genotypic 
      subpopulations.
FAU - Balazs, M
AU  - Balazs M
AD  - Department of Laboratory Medicine, University of California San Francisco 
      94143-0808, USA.
FAU - Matsumura, K
AU  - Matsumura K
FAU - Moore, D
AU  - Moore D
FAU - Pinkel, D
AU  - Pinkel D
FAU - Gray, J W
AU  - Gray JW
FAU - Waldman, F M
AU  - Waldman FM
LA  - eng
GR  - CA-44768/CA/NCI NIH HHS/United States
GR  - CA-45919/CA/NCI NIH HHS/United States
GR  - CA-49056/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
SB  - IM
MH  - Adult
MH  - Aged
MH  - Breast Neoplasms/chemistry/*genetics/pathology
MH  - Carcinoma in Situ/chemistry/*genetics/pathology
MH  - Carcinoma, Ductal, Breast/chemistry/*genetics/pathology
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - *Karyotyping
MH  - Middle Aged
MH  - Ploidies
EDAT- 1995/05/01 00:00
MHDA- 1995/05/01 00:01
CRDT- 1995/05/01 00:00
PHST- 1995/05/01 00:00 [pubmed]
PHST- 1995/05/01 00:01 [medline]
PHST- 1995/05/01 00:00 [entrez]
AID - 10.1002/cyto.990200110 [doi]
PST - ppublish
SO  - Cytometry. 1995 May 1;20(1):62-73. doi: 10.1002/cyto.990200110.