PMID- 7600984
OWN - NLM
STAT- MEDLINE
DCOM- 19950810
LR  - 20220215
IS  - 0950-1991 (Print)
IS  - 0950-1991 (Linking)
VI  - 121
IP  - 6
DP  - 1995 Jun
TI  - TGF alpha can act as a chemoattractant to perioptic mesenchymal cells in 
      developing mouse eyes.
PG  - 1669-80
AB  - Growth factors are believed to play an important role in regulating cell fate and 
      cell behavior during embryonic development. Transforming growth factor alpha (TGF 
      alpha), a member of the epidermal growth factor (EGF) superfamily, is a small 
      polypeptide growth factor. Upon binding to its receptor, the EGF receptor (EGFR), 
      TGF alpha can exert diverse biological activities, such as induction of cell 
      proliferation or differentiation. To explore the possibility that TGF alpha might 
      regulate cell fate during murine eye development, we generated transgenic mice 
      that express human TGF alpha in the lens under the control of the mouse alpha 
      A-crystallin promoter. The transgenic mice displayed multiple eye defects, 
      including corneal opacities, cataracts and microphthalmia. At early embryonic 
      stages TGF alpha induced the perioptic mesenchymal cells to migrate abnormally 
      into the eye and accumulate around the lens. In situ hybridization revealed that 
      the EGFR mRNA is highly expressed in the perioptic mesenchyme, suggesting that 
      the migratory response is mediated by receptor activation. In order to test this 
      model, the TGF alpha transgenic mice were bred to EGFR mutant waved-2 (wa-2) 
      mice. We found that the eye defects of the TGF alpha transgenic mice are 
      significantly abated in the wa-2 homozygote background. Because the EGFR mutation 
      in the wa-2 mice is located in the receptor kinase domain, this result indicates 
      that the receptor tyrosine kinase activity is critical for signaling the 
      migratory response. Taken together, our studies demonstrate that TGF alpha is 
      capable of altering the migratory decisions and behavior of perioptic mesenchyme 
      during eye development.
FAU - Reneker, L W
AU  - Reneker LW
AD  - Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030, USA.
FAU - Silversides, D W
AU  - Silversides DW
FAU - Patel, K
AU  - Patel K
FAU - Overbeek, P A
AU  - Overbeek PA
LA  - eng
GR  - EY-06578/EY/NEI NIH HHS/United States
GR  - EY-10448/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Development
JT  - Development (Cambridge, England)
JID - 8701744
RN  - 0 (Chemotactic Factors)
RN  - 0 (DNA Primers)
RN  - 0 (Transforming Growth Factor alpha)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Movement
MH  - *Chemotactic Factors
MH  - DNA Primers/genetics
MH  - ErbB Receptors/metabolism
MH  - Eye/anatomy & histology/*embryology
MH  - In Situ Hybridization
MH  - Lens, Crystalline/embryology
MH  - Mesoderm/*cytology
MH  - Mice
MH  - Mice, Transgenic
MH  - Molecular Sequence Data
MH  - Morphogenesis
MH  - Phenotype
MH  - Polymerase Chain Reaction
MH  - Transforming Growth Factor alpha/metabolism/*physiology
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
AID - 10.1242/dev.121.6.1669 [doi]
PST - ppublish
SO  - Development. 1995 Jun;121(6):1669-80. doi: 10.1242/dev.121.6.1669.