PMID- 7601632
OWN - NLM
STAT- MEDLINE
DCOM- 19950807
LR  - 20100324
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 36
IP  - 8
DP  - 1995 Jul
TI  - Keratinocyte growth factor accelerates corneal epithelial wound healing in vivo.
PG  - 1524-9
AB  - PURPOSE: To examine whether the topical application of keratinocyte growth factor 
      (KGF) can enhance corneal epithelial healing in vivo. In addition, the 
      distribution of S-phase cells in KGF-treated and control corneas was investigated 
      during regeneration and under normal conditions. METHODS: A 10-mm diameter 
      epithelial defect was made in the center of rabbit corneas. A 50-microliters 
      aliquot of 10 micrograms/ml human keratinocyte growth factor (hKGF) was then 
      applied topically five times a day. The same volume of phosphate-buffered saline 
      (PBS) vehicle was applied to the contralateral eye as a control. Each corneal 
      epithelial defect was subsequently photographed every 12 hours and was measured 
      by a computer-assisted digitizer. For the S-phase cell analysis, entire corneas 
      were labeled with 3H-thymidine and were subjected to autoradiography at 24 hours 
      after wounding or in the normal cornea at 24 hours after the application of KGF 
      or PBS. RESULTS: Topical application of 10 micrograms/ml hKGF significantly 
      accelerated corneal epithelial wound healing when compared with controls. In the 
      S-phase cell analysis, KGF did not have any effect on normal corneal epithelial 
      cells. However, in the regenerating cornea, the number of S-phase cells in the 
      KGF-treated limbal epithelium was twofold higher than in the controls. 
      CONCLUSIONS: Topical application of KGF accelerated corneal epithelial wound 
      healing in vivo and increased cell proliferation in the limbal epithelium of the 
      regenerating cornea.
FAU - Sotozono, C
AU  - Sotozono C
AD  - Department of Ophthalmology, Kyoto Prefectural University of Medicine, Japan.
FAU - Inatomi, T
AU  - Inatomi T
FAU - Nakamura, M
AU  - Nakamura M
FAU - Kinoshita, S
AU  - Kinoshita S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Fibroblast Growth Factor 10)
RN  - 0 (Growth Substances)
RN  - 0 (Ophthalmic Solutions)
RN  - 0 (Recombinant Proteins)
RN  - 126469-10-1 (Fibroblast Growth Factor 7)
RN  - 62031-54-3 (Fibroblast Growth Factors)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Administration, Topical
MH  - Animals
MH  - Autoradiography
MH  - Cell Division
MH  - Cornea/cytology/drug effects/*physiology
MH  - DNA/biosynthesis
MH  - DNA Replication/drug effects/physiology
MH  - Epithelium/drug effects/physiology
MH  - Fibroblast Growth Factor 10
MH  - Fibroblast Growth Factor 7
MH  - *Fibroblast Growth Factors
MH  - Growth Substances/*pharmacology
MH  - Ophthalmic Solutions
MH  - Rabbits
MH  - Recombinant Proteins
MH  - S Phase/drug effects/physiology
MH  - Wound Healing/*drug effects
EDAT- 1995/07/01 00:00
MHDA- 1995/07/01 00:01
CRDT- 1995/07/01 00:00
PHST- 1995/07/01 00:00 [pubmed]
PHST- 1995/07/01 00:01 [medline]
PHST- 1995/07/01 00:00 [entrez]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 1995 Jul;36(8):1524-9.