PMID- 7602238
OWN - NLM
STAT- MEDLINE
DCOM- 19950804
LR  - 20131121
IS  - 0022-2143 (Print)
IS  - 0022-2143 (Linking)
VI  - 126
IP  - 1
DP  - 1995 Jul
TI  - Modulation of neutrophil function by novel colonic factors: possible role in the 
      pathophysiology of ulcerative colitis.
PG  - 70-80
AB  - Tissue damage in acute ulcerative colitis (UC) may be triggered by neutrophils 
      (PMNs) and their inflammatory mediators such as reactive oxygen species (ROS). 
      Because circulating PMNs appeared normal in subjects with UC, we hypothesized 
      that the critical abnormality that attracts and activates PMNs in UC is a local 
      colonic factor. Accordingly, the colonic milieu was sampled by using in vivo 
      rectal dialysis (mol wt < or = 12 kd). Normal PMNs were exposed in vitro to 
      rectal dialysates (RD) from control subjects (cRD) or subjects with active UC 
      (aRD) or inactive UC (IRD). PMN-derived ROS were measured by chemiluminescence. 
      cRD did not increase ROS production by unstimulated PMNs; aRD significantly and 
      concentration-dependently increased ROS; IRD gave intermediate results. cRD 
      inhibited the PMN-stimulating effects of both the bacterial peptide 
      formyl-methionyl-leucyl-phenylalanine (fMLP) and phorbol myristate acetate (PMA). 
      aRD and IRD blunted the effect of fMLP and PMA significantly less than did cRD. 
      Rectal dialysates from 44% of subjects with active UC exaggerated the fMLP 
      effect, whereas potentiation occurred for only 13% of cRDs and 18% of iRDs. cRD 
      preconditioned with either activated or nonactivated PMNs was not significantly 
      different than unconditioned cRD. We thus infer the existence of colonic factors 
      in UC that (1) can trigger PMNs to produce ROS and (2) have a proinflammatory 
      modulatory effect on bacterial peptide-induced, PMN-mediated ROS production, 
      thereby initiating or perpetuating inflammation and eventually causing tissue 
      damage.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Kazi, N
AU  - Kazi N
AD  - Department of Medicine, School of Medicine, Loyola University at Chicago, 
      Maywood, IL 60153, USA.
FAU - Fields, J Z
AU  - Fields JZ
FAU - Sedghi, S
AU  - Sedghi S
FAU - Kottapalli, V
AU  - Kottapalli V
FAU - Eiznhamer, D
AU  - Eiznhamer D
FAU - Winship, D
AU  - Winship D
FAU - Keshavarzian, A
AU  - Keshavarzian A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Lab Clin Med
JT  - The Journal of laboratory and clinical medicine
JID - 0375375
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 5EXP385Q4F (Luminol)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Colitis, Ulcerative/*physiopathology
MH  - Colon/*metabolism
MH  - Dialysis
MH  - Female
MH  - Humans
MH  - Luminescent Measurements
MH  - Luminol/pharmacology
MH  - Male
MH  - Middle Aged
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*physiology
MH  - Rectum
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 1995/07/01 00:00
MHDA- 1995/07/01 00:01
CRDT- 1995/07/01 00:00
PHST- 1995/07/01 00:00 [pubmed]
PHST- 1995/07/01 00:01 [medline]
PHST- 1995/07/01 00:00 [entrez]
PST - ppublish
SO  - J Lab Clin Med. 1995 Jul;126(1):70-80.