PMID- 7604875
OWN - NLM
STAT- MEDLINE
DCOM- 19950808
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 147
IP  - 1
DP  - 1995 Jul
TI  - Detection of numerical alterations for chromosomes 7 and 12 in benign thyroid 
      lesions by in situ hybridization. Histological implications.
PG  - 136-44
AB  - Polysomies of chromosomes 7 and 12 have been frequently observed by conventional 
      cytogenetics in a subgroup of thyroid follicular adenomas and in some cases of 
      thyroid goiters. To further study possible cytogenetic similarities between these 
      two types of thyroid lesions, we have used fluorescence in situ hybridization 
      (FISH) to detect polysomies of chromosomes 7 and/or 12 in isolated nuclei from 
      frozen and paraffin-embedded material of goiters and thyroid follicular adenomas 
      and compared results with previous ones obtained by flow cytometry and 
      conventional cytogenetics. With a set of two alpha-satellite DNA probes specific 
      for the centromeric regions of chromosomes 7 and 12, used either separately 
      (single-target fluorescence in situ hybridization) or simultaneously 
      (double-target fluorescence in situ hybridization), we detected polysomies of 
      chromosome 7 in 35.7% of the thyroid follicular adenomas and in 10.7% of the 
      goiters. Polysomies of chromosome 12 were detected in 29.6% of the thyroid 
      follicular adenomas and 6.7% of the goiters. The significantly higher frequency 
      of adenomas with numerical alterations for chromosomes 7 and/or 12 supports the 
      idea of a biological continuum and karyotypic evolution between both lesions. It 
      is also noteworthy that polysomies of chromosomes 7 and/or 12 were observed only 
      in lesions with an exclusive (or predominant) microfollicular histological 
      component, as detected by enzymatic in situ hybridization on frozen sections.
FAU - Criado, B
AU  - Criado B
AD  - Unit of Genetics, IPATIMUP, Medical Faculty of Porto, Portugal.
FAU - Barros, A
AU  - Barros A
FAU - Suijkerbuijk, R F
AU  - Suijkerbuijk RF
FAU - Weghuis, D O
AU  - Weghuis DO
FAU - Seruca, R
AU  - Seruca R
FAU - Fonseca, E
AU  - Fonseca E
FAU - Castedo, S
AU  - Castedo S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adenoma/diagnosis/*genetics
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Chromosome Aberrations/*diagnosis
MH  - Chromosome Disorders
MH  - *Chromosomes, Human, Pair 12
MH  - *Chromosomes, Human, Pair 7
MH  - DNA Probes
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - Goiter/diagnosis/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Ploidies
MH  - Thyroid Neoplasms/diagnosis/*genetics
PMC - PMC1869887
EDAT- 1995/07/01 00:00
MHDA- 1995/07/01 00:01
PMCR- 1996/01/01
CRDT- 1995/07/01 00:00
PHST- 1995/07/01 00:00 [pubmed]
PHST- 1995/07/01 00:01 [medline]
PHST- 1995/07/01 00:00 [entrez]
PHST- 1996/01/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1995 Jul;147(1):136-44.