PMID- 7606359
OWN - NLM
STAT- MEDLINE
DCOM- 19950817
LR  - 20191031
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 3
IP  - 4
DP  - 1995 Jun
TI  - Microdissection of the Y chromosome and fluorescence in situ hybridization 
      analysis of the sex chromosomes of lake trout, Salvelinus namaycush.
PG  - 221-6
AB  - Lake trout, Salvelinus namaycush, is one of the few salmonids with 
      morphologically differentiated sex chromosomes. Genetic analysis suggested that 
      the sex-determining region of this species lies on the short arm of the Y 
      chromosome. The differential arm of the Y chromosome was microdissected and the 
      resulting DNA amplified in a sequence-independent manner. Amplified DNA was 
      biotin labeled as a probe for fluorescence in situ hybridization (FISH). Strong 
      hybridization signals were seen covering defined regions of both the Y and X 
      chromosomes. Homeologous chromosomes of the ancestrally tetraploid genome were 
      not identified by FISH with the Y probe, indicating diploidization of this region 
      of the genome.
FAU - Reed, K M
AU  - Reed KM
AD  - Department of Biological Sciences, University of Wisconsin-Milwaukee 53201, USA.
FAU - Bohlander, S K
AU  - Bohlander SK
FAU - Phillips, R B
AU  - Phillips RB
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (DNA Probes)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Chromosomes
MH  - DNA Probes
MH  - Female
MH  - Genetic Techniques
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Molecular Sequence Data
MH  - Trout/*genetics
MH  - *X Chromosome
MH  - *Y Chromosome
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
AID - 10.1007/BF00713046 [doi]
PST - ppublish
SO  - Chromosome Res. 1995 Jun;3(4):221-6. doi: 10.1007/BF00713046.