PMID- 7606922
OWN - NLM
STAT- MEDLINE
DCOM- 19950816
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 71
IP  - 1
DP  - 1995
TI  - Improved simple generation of GTG-band specific painting probes.
PG  - 32-6
AB  - We developed an improved, simple method of generating chromosome-region-specific 
      probes from only a few microdissected chromosomes. One to five dissected 
      fragments from a defined chromosomal region were processed with a PEG/proteinase 
      K cycling deproteinization step and directly amplified with a two-step 
      amplification system using a degenerate oligonucleotide primed shuttle polymerase 
      chain reaction (DOP-Shuttle-PCR). This modified method offered three advantages 
      over previously reported methods: relaxation of the highly condensed chromosomal 
      DNA, reduction of the risk of endogenous and exogenous contamination, and high 
      efficiency amplification of template DNA. High intensity in the fluorescence in 
      situ hybridization (FISH) signals from normal metaphase chromosomes, as well as 
      regional specificity of these probes, corresponding to regions on R-banded 
      chromosomes, were observed.
FAU - Yokoyama, Y
AU  - Yokoyama Y
AD  - Department of Chromosome Analysis, Center for Molecular Genetics, Hachioji, 
      Japan.
FAU - Sakuragawa, N
AU  - Sakuragawa N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (DNA Primers)
RN  - 0 (DNA Probes)
RN  - 0 (Oligonucleotides)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.64 (Endopeptidase K)
SB  - IM
MH  - Base Sequence
MH  - Cell Line, Transformed
MH  - Chromosome Banding/*methods
MH  - DNA Primers
MH  - *DNA Probes
MH  - Endopeptidase K
MH  - Humans
MH  - Molecular Sequence Data
MH  - Oligonucleotides
MH  - Polymerase Chain Reaction/methods
MH  - Serine Endopeptidases
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1159/000134056 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1995;71(1):32-6. doi: 10.1159/000134056.