PMID- 7606937 OWN - NLM STAT- MEDLINE DCOM- 19950816 LR - 20180214 IS - 0301-0171 (Print) IS - 0301-0171 (Linking) VI - 71 IP - 1 DP - 1995 TI - Simultaneous visualization of Q-bands and FISH signals using a novel fluorochrome. PG - 96-8 AB - We developed a new HNPP-azo dye method for detection of fluorescence in situ hybridization (FISH) signals on Q-banded chromosomes by use of a newly synthesized fluorochrome, HNPP (3-hydroxy-N-2'-biphenyl-2-naphthalenecarboxamide phosphate ester), which reacts enzymatically with alkaline phosphatase and azo dye. The FISH staining method permits simultaneous detection of orange HNPP signals on chromosomal sites labeled by Q-banding, allowing the assignment of small (440-1,200 bp) probes. FAU - Kondoh, Y AU - Kondoh Y AD - Aisin Cosmos R&D Co., Ltd., Kariya, Aichi, Japan. FAU - Ono, T AU - Ono T FAU - Kagiyama, N AU - Kagiyama N FAU - Fujita, S AU - Fujita S FAU - Momiyama, M AU - Momiyama M FAU - Hori, S H AU - Hori SH FAU - Yoshida, M C AU - Yoshida MC LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Switzerland TA - Cytogenet Cell Genet JT - Cytogenetics and cell genetics JID - 0367735 RN - 0 (Azo Compounds) RN - 0 (Fluorescent Dyes) RN - 0 (Naphthalenes) RN - 140446-67-9 (3-hydroxy-N-(2'-biphenyl)-2-naphthalenecarboxamide phosphate) SB - IM MH - Animals MH - Azo Compounds MH - Cells, Cultured MH - Chromosome Banding/*methods MH - *Fluorescent Dyes MH - Humans MH - In Situ Hybridization, Fluorescence/*methods MH - Mice MH - *Naphthalenes EDAT- 1995/01/01 00:00 MHDA- 1995/01/01 00:01 CRDT- 1995/01/01 00:00 PHST- 1995/01/01 00:00 [pubmed] PHST- 1995/01/01 00:01 [medline] PHST- 1995/01/01 00:00 [entrez] AID - 10.1159/000134071 [doi] PST - ppublish SO - Cytogenet Cell Genet. 1995;71(1):96-8. doi: 10.1159/000134071.